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MICAL1 Polyclonal Antibody

MICAL1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 27

MICAL1 Antibody (14818-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 14818-1-AP (MICAL1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

MICAL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MICAL1 Antibody in Flow Cytometry (Flow)

MICAL1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14818-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MICAL1 Fusion Protein Ag6578 (718-1067 aa encoded by BC052983)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody specifically recognizes MICAL1.

Immunogen sequence: HRSCFRCHT CEATLWPGGY EQHPGDGHFY CLQHLPQTDH KAEGSDRGPE SPELPTPSEN SMPPGLSTPT ASQEGAGPVP DPSQPTRRQI RLSSPERQRL SSLNLTPDPE MEPPPKPPRS CSALARHALE SSFVGWGLPV QSPQALVAME KEEKESPFSS EEEEEDVPLD SDVEQALQTF AKTSGTMNNY PTWRRTLLRR AKEEEMKRFC KAQTIQRRLN EIEAALRELE AEGVKLELAL RRQSSSPEQQ KKLWVGQLLQ LVDKKNSLVA EEAELMITVQ ELNLEEKQWQ LDQELRGYMN REENLKTAAD RQAEDQVLRK LVDLVNQRDA LIRFQEERRL SELALGTGAQ G (718-1067 aa encoded by BC052983)

Target Information

May be a cytoskeletal regulator that connects NEDD9 to intermediate filaments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High MICAL1 expression correlates with cancer progression and immune infiltration in renal clear cell carcinoma.

BMC cancer

Yang Y,Ye F,Xia T,Wang Q,Du J

Tue Dec 27 00:00:00 EST 2022

High MICAL1 expression correlates with cancer progression and immune infiltration in renal clear cell carcinoma.

BMC cancer

Yang Y,Ye F,Xia T,Wang Q,Du J

Tue Dec 27 00:00:00 EST 2022

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement.

Oncogene

Zhang Z,Liu R,Wang Y,Wang Y,Shuai Y,Ke C,Jin R,Wang X,Luo J

Sat Jan 01 00:00:00 EST 2022

MICAL1 inhibits colorectal cancer cell migration and proliferation by regulating the EGR1/β-catenin signaling pathway.

Biochemical pharmacology

Gu H,Li Y,Cui X,Cao H,Hou Z,Ti Y,Liu D,Gao J,Wang Y,Wen P

Sat Jan 01 00:00:00 EST 2022

MICAL1 inhibits colorectal cancer cell migration and proliferation by regulating the EGR1/β-catenin signaling pathway.

Biochemical pharmacology

Gu H,Li Y,Cui X,Cao H,Hou Z,Ti Y,Liu D,Gao J,Wang Y,Wen P

Sat Jan 01 00:00:00 EST 2022

PlexinA1-deficient mice exhibit decreased cell density and augmented oxidative stress in parvalbumin-expressing interneurons in the medial prefrontal cortex.

IBRO neuroscience reports

Jahan MS,Tsuzuki T,Ito T,Bhuiyan MER,Takahashi I,Takamatsu H,Kumanogoh A,Negishi T,Yukawa K

Thu Dec 01 00:00:00 EST 2022

Mical modulates Tau toxicity via cysteine oxidation in vivo.

Acta neuropathologica communications

Prifti E,Tsakiri EN,Vourkou E,Stamatakis G,Samiotaki M,Skoulakis EMC,Papanikolopoulou K

Mon Apr 04 00:00:00 EDT 2022

Mical modulates Tau toxicity via cysteine oxidation in vivo.

Acta neuropathologica communications

Prifti E,Tsakiri EN,Vourkou E,Stamatakis G,Samiotaki M,Skoulakis EMC,Papanikolopoulou K

Mon Apr 04 00:00:00 EDT 2022

MICAL2 enhances branched actin network disassembly by oxidizing Arp3B-containing Arp2/3 complexes.

The Journal of cell biology

Galloni C,Carra D,Abella JVG,Kjær S,Singaravelu P,Barry DJ,Kogata N,Guérin C,Blanchoin L,Way M

Mon Aug 02 00:00:00 EDT 2021

Rab11FIP1 maintains Rab35 at the intercellular bridge to promote actin removal and abscission.

Journal of cell science

Iannantuono NVG,Emery G

Tue Jun 15 00:00:00 EDT 2021

MICAL1 (molecule interacting with CasL 1) protects oligodendrocyte cells from oxidative injury through regulating apoptosis, autophagy in spinal cord injury.

Neuroscience letters

Xu C,Mao L,Tian H,Lin S,Zhao X,Lin J,Li D,Li X,Mei X

Sat Apr 17 00:00:00 EDT 2021

MICAL1 regulates actin cytoskeleton organization, directional cell migration and the growth of human breast cancer cells as orthotopic xenograft tumours.

Cancer letters

McGarry DJ,Armstrong G,Castino G,Mason S,Clark W,Shaw R,McGarry L,Blyth K,Olson MF

Thu Oct 28 00:00:00 EDT 2021

F-actin disassembly factor MICAL1 binding to Myosin Va mediates cargo unloading during cytokinesis.

Science advances

Niu F,Sun K,Wei W,Yu C,Wei Z

Sun Nov 01 00:00:00 EDT 2020

TBC1D1 interacting proteins, VPS13A and VPS13C, regulate GLUT4 homeostasis in C2C12 myotubes.

Scientific reports

Hook SC,Chadt A,Heesom KJ,Kishida S,Al-Hasani H,Tavaré JM,Thomas EC

Wed Oct 21 00:00:00 EDT 2020

NEDD9 Facilitates Hypoxia-Induced Gastric Cancer Cell Migration via MICAL1 Related Rac1 Activation.

Frontiers in pharmacology

Zhao S,Min P,Liu L,Zhang L,Zhang Y,Wang Y,Zhao X,Ma Y,Xie H,Zhu C,Jiang H,Du J,Gu L

Sun Apr 10 00:00:00 EDT 2022

NEDD9 Facilitates Hypoxia-Induced Gastric Cancer Cell Migration via MICAL1 Related Rac1 Activation.

Frontiers in pharmacology

Zhao S,Min P,Liu L,Zhang L,Zhang Y,Wang Y,Zhao X,Ma Y,Xie H,Zhu C,Jiang H,Du J,Gu L

Sun Apr 10 00:00:00 EDT 2022

MICAL1 facilitates breast cancer cell proliferation via ROS-sensitive ERK/cyclin D pathway.

Journal of cellular and molecular medicine

Deng W,Wang Y,Zhao S,Zhang Y,Chen Y,Zhao X,Liu L,Sun S,Zhang L,Ye B,Du J

Fri Jun 01 00:00:00 EDT 2018

MICAL1 facilitates breast cancer cell proliferation via ROS-sensitive ERK/cyclin D pathway.

Journal of cellular and molecular medicine

Deng W,Wang Y,Zhao S,Zhang Y,Chen Y,Zhao X,Liu L,Sun S,Zhang L,Ye B,Du J

Fri Jun 01 00:00:00 EDT 2018

NEDD9 stimulated MMP9 secretion is required for invadopodia formation in oral squamous cell carcinoma.

Oncotarget

Grauzam S,Brock AM,Holmes CO,Tiedeken JA,Boniface SG,Pierson BN,Patterson DG,Coaxum SD,Neskey DM,Rosenzweig SA

Tue May 22 00:00:00 EDT 2018

NEDD9 stimulated MMP9 secretion is required for invadopodia formation in oral squamous cell carcinoma.

Oncotarget

Grauzam S,Brock AM,Holmes CO,Tiedeken JA,Boniface SG,Pierson BN,Patterson DG,Coaxum SD,Neskey DM,Rosenzweig SA

Tue May 22 00:00:00 EDT 2018

NEDD9 stimulated MMP9 secretion is required for invadopodia formation in oral squamous cell carcinoma.

Oncotarget

Grauzam S,Brock AM,Holmes CO,Tiedeken JA,Boniface SG,Pierson BN,Patterson DG,Coaxum SD,Neskey DM,Rosenzweig SA

Tue May 22 00:00:00 EDT 2018

NEDD9 stimulated MMP9 secretion is required for invadopodia formation in oral squamous cell carcinoma.

Oncotarget

Grauzam S,Brock AM,Holmes CO,Tiedeken JA,Boniface SG,Pierson BN,Patterson DG,Coaxum SD,Neskey DM,Rosenzweig SA

Tue May 22 00:00:00 EDT 2018

Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling.

Developmental cell

Yoon J,Kim SB,Ahmed G,Shay JW,Terman JR

Mon Jul 24 00:00:00 EDT 2017

Amplification of F-Actin Disassembly and Cellular Repulsion by Growth Factor Signaling.

Developmental cell

Yoon J,Kim SB,Ahmed G,Shay JW,Terman JR

Mon Jul 24 00:00:00 EDT 2017

Oxidation of F-actin controls the terminal steps of cytokinesis.

Nature communications

Frémont S,Hammich H,Bai J,Wioland H,Klinkert K,Rocancourt M,Kikuti C,Stroebel D,Romet-Lemonne G,Pylypenko O,Houdusse A,Echard A

Thu Feb 23 00:00:00 EST 2017

Oxidation of F-actin controls the terminal steps of cytokinesis.

Nature communications

Frémont S,Hammich H,Bai J,Wioland H,Klinkert K,Rocancourt M,Kikuti C,Stroebel D,Romet-Lemonne G,Pylypenko O,Houdusse A,Echard A

Thu Feb 23 00:00:00 EST 2017

Podocyte Shape Regulation by Semaphorin 3A and MICAL-1.

Methods in molecular biology (Clifton, N.J.)

Tufro A

Thu Feb 15 00:00:00 EST 2018

Podocyte Shape Regulation by Semaphorin 3A and MICAL-1.

Methods in molecular biology (Clifton, N.J.)

Tufro A

Thu Feb 15 00:00:00 EST 2018

MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells.

BMC cancer

Deng W,Wang Y,Gu L,Duan B,Cui J,Zhang Y,Chen Y,Sun S,Dong J,Du J

Mon Jul 18 00:00:00 EDT 2016

MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells.

BMC cancer

Deng W,Wang Y,Gu L,Duan B,Cui J,Zhang Y,Chen Y,Sun S,Dong J,Du J

Mon Jul 18 00:00:00 EDT 2016

MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells.

BMC cancer

Deng W,Wang Y,Gu L,Duan B,Cui J,Zhang Y,Chen Y,Sun S,Dong J,Du J

Mon Jul 18 00:00:00 EDT 2016

MICAL1 controls cell invasive phenotype via regulating oxidative stress in breast cancer cells.

BMC cancer

Deng W,Wang Y,Gu L,Duan B,Cui J,Zhang Y,Chen Y,Sun S,Dong J,Du J

Mon Jul 18 00:00:00 EDT 2016

Semaphorin3a promotes advanced diabetic nephropathy.

Diabetes

Aggarwal PK,Veron D,Thomas DB,Siegel D,Moeckel G,Kashgarian M,Tufro A

Fri May 01 00:00:00 EDT 2015

Semaphorin3a promotes advanced diabetic nephropathy.

Diabetes

Aggarwal PK,Veron D,Thomas DB,Siegel D,Moeckel G,Kashgarian M,Tufro A

Fri May 01 00:00:00 EDT 2015

Differential regulation of actin microfilaments by human MICAL proteins.

Journal of cell science

Giridharan SS,Rohn JL,Naslavsky N,Caplan S

Wed Feb 01 00:00:00 EST 2012

Differential regulation of actin microfilaments by human MICAL proteins.

Journal of cell science

Giridharan SS,Rohn JL,Naslavsky N,Caplan S

Wed Feb 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: [F-actin]-monooxygenase MICAL1; CasL interacting molecule MICAL; MICAL-1; microtubule associated monoxygenase, calponin and LIM domain containing 1; mMical1; Molecule interacting with CasL protein 1; NEDD9 interacting protein with calponin homology and LIM domains; NEDD9-interacting protein with calponin homology and LIM domains; protein-methionine sulfoxide oxidase MICAL1

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Gene Aliases: MICAL; MICAL-1; MICAL1; NICAL

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UniProt ID: (Human) Q8TDZ2, (Mouse) Q8VDP3

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Entrez Gene ID: (Human) 64780, (Mouse) 171580

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Function(s)

Process(es)

negative regulation of protein phosphorylation cytoskeleton organization signal transduction sulfur oxidation actin filament depolymerization negative regulation of cysteine-type endopeptidase activity involved in apoptotic process oxidation-reduction process negative regulation of apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MICAL1 Polyclonal Antibody

MICAL1 Antibody (14818-1-AP) in ICC/IF

Product Details

14818-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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