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MICAL2 Polyclonal Antibody

MICAL2 Antibody in Immunocytochemistry (ICC/IF)

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MICAL2 Antibody (13965-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using MICAL2 antibody (Product # 13965-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MICAL2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MICAL2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

13965-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MICAL2 Fusion Protein Ag4950 (871-1124 aa encoded by BC044577)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody can recognize both MICAL2 and MICAL2-PV. This antibody recognizes various isoforms of MICAL2 around 90-95 kDa or 109-112 kDa.

Immunogen sequence: KAAHLASMF GHGDFPQNKL LSKGLSHTHP PSPPSRLPSP DPAASSSPST VDSASPARKE KKSPSGFHFH PSHLRTVHPQ LTVGKVSSGI GAAAEVLVNL YMNDHRPKAQ ATSPDLESMR KSFPLNLGGS DTCYFCKKRV YVMERLSAEG HFFHRECFRC SICATTLRLA AYTFDCDEGK FYCKPHFIHC KTNSKQRKRR AELKQQREEE ATWQEQEAPR RDTPTESSCA VAAIGTLEGS PPVHFSLPVL HPLLG (871-1124 aa encoded by BC044577)

Target Information

MICALs (Molecules Interacting with CasL) are atypical multidomain flavoenzymes with diverse cellular functions. There are three known isoforms, MICAL1, MICAL2 and MICAL3, as well as the MICAL-like proteins MICAL-L1 and MICAL-L2. MICAL2 has three conserved domains: an N-terminal flavin adenine dinucleotide (FAD) binding domain, a calponin homology (CH) domain and a Lin11, Isl-1 and Mec-3 (LIM) domain. It has been demonstrated that MICAL2 could regulate actin stress fibers and is required for normal actin organization. In addition, MICAL2-PV, a novel splicing variants of MICAL2, has been reported to be involved in cancer progression of prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement.

Oncogene

Zhang Z,Liu R,Wang Y,Wang Y,Shuai Y,Ke C,Jin R,Wang X,Luo J

Sat Jan 01 00:00:00 EST 2022

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement.

Oncogene

Zhang Z,Liu R,Wang Y,Wang Y,Shuai Y,Ke C,Jin R,Wang X,Luo J

Sat Jan 01 00:00:00 EST 2022

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement.

Oncogene

Zhang Z,Liu R,Wang Y,Wang Y,Shuai Y,Ke C,Jin R,Wang X,Luo J

Sat Jan 01 00:00:00 EST 2022

Phosphorylation of MICAL2 by ARG promotes head and neck cancer tumorigenesis by regulating skeletal rearrangement.

Oncogene

Zhang Z,Liu R,Wang Y,Wang Y,Shuai Y,Ke C,Jin R,Wang X,Luo J

Sat Jan 01 00:00:00 EST 2022

Exosome-like nanovesicles derived from Phellinus linteus inhibit Mical2 expression through cross-kingdom regulation and inhibit ultraviolet-induced skin aging.

Journal of nanobiotechnology

Han J,Wu T,Jin J,Li Z,Cheng W,Dai X,Yang K,Zhang H,Zhang Z,Zhang H,Fan R,Zheng S,Liu H,Li Y,Zhao H,Yao C,Lin T,Zhu C,Liu H

Fri Oct 21 00:00:00 EDT 2022

Exosome-like nanovesicles derived from Phellinus linteus inhibit Mical2 expression through cross-kingdom regulation and inhibit ultraviolet-induced skin aging.

Journal of nanobiotechnology

Han J,Wu T,Jin J,Li Z,Cheng W,Dai X,Yang K,Zhang H,Zhang Z,Zhang H,Fan R,Zheng S,Liu H,Li Y,Zhao H,Yao C,Lin T,Zhu C,Liu H

Fri Oct 21 00:00:00 EDT 2022

Exosome-like nanovesicles derived from Phellinus linteus inhibit Mical2 expression through cross-kingdom regulation and inhibit ultraviolet-induced skin aging.

Journal of nanobiotechnology

Han J,Wu T,Jin J,Li Z,Cheng W,Dai X,Yang K,Zhang H,Zhang Z,Zhang H,Fan R,Zheng S,Liu H,Li Y,Zhao H,Yao C,Lin T,Zhu C,Liu H

Fri Oct 21 00:00:00 EDT 2022

Exosome-like nanovesicles derived from Phellinus linteus inhibit Mical2 expression through cross-kingdom regulation and inhibit ultraviolet-induced skin aging.

Journal of nanobiotechnology

Han J,Wu T,Jin J,Li Z,Cheng W,Dai X,Yang K,Zhang H,Zhang Z,Zhang H,Fan R,Zheng S,Liu H,Li Y,Zhao H,Yao C,Lin T,Zhu C,Liu H

Fri Oct 21 00:00:00 EDT 2022

A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis.

Cell death & disease

Tan Y,Jiang C,Jia Q,Wang J,Huang G,Tang F

Sat Apr 23 00:00:00 EDT 2022

A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis.

Cell death & disease

Tan Y,Jiang C,Jia Q,Wang J,Huang G,Tang F

Sat Apr 23 00:00:00 EDT 2022

MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation.

Oxidative medicine and cellular longevity

Qi C,Min P,Wang Q,Wang Y,Song Y,Zhang Y,Bibi M,Du J

Mon Feb 07 00:00:00 EST 2022

MICAL2 Facilitates Gastric Cancer Cell Migration via MRTF-A-Mediated CDC42 Activation.

Frontiers in molecular biosciences

Wang Y,Min P,Qi C,Zhao S,Yu M,Zhang Y,Du J

Tue Apr 13 00:00:00 EDT 2021

MICAL2 is a novel nucleocytoplasmic shuttling protein promoting cancer invasion and growth of lung adenocarcinoma.

Cancer letters

Zhou W,Liu Y,Gao Y,Cheng Y,Chang R,Li X,Zhou Y,Wang S,Liang L,Duan C,Zhang C

Tue Jul 28 00:00:00 EDT 2020

Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction.

Theranostics

Xu C,Zhou X,Xie T,Zhou Y,Zhang Q,Jiang S,Zhang R,Liao L,Dong J

Tue May 18 00:00:00 EDT 2021

miR-205-5p suppresses pulmonary vascular smooth muscle cell proliferation by targeting MICAL2-mediated Erk1/2 signaling.

Microvascular research

Tao W,Sun W,Zhu H,Zhang J

Mon Jul 01 00:00:00 EDT 2019

MICAL2 promotes breast cancer cell migration by maintaining epidermal growth factor receptor (EGFR) stability and EGFR/P38 signalling activation.

Acta physiologica (Oxford, England)

Wang Y,Deng W,Zhang Y,Sun S,Zhao S,Chen Y,Zhao X,Liu L,Du J

Thu Feb 01 00:00:00 EST 2018

MICAL2 promotes breast cancer cell migration by maintaining epidermal growth factor receptor (EGFR) stability and EGFR/P38 signalling activation.

Acta physiologica (Oxford, England)

Wang Y,Deng W,Zhang Y,Sun S,Zhao S,Chen Y,Zhao X,Liu L,Du J

Thu Feb 01 00:00:00 EST 2018

RNA Sequencing of Carboplatin- and Paclitaxel-Resistant Endometrial Cancer Cells Reveals New Stratification Markers and Molecular Targets for Cancer Treatment.

Hormones & cancer

Hellweg R,Mooneyham A,Chang Z,Shetty M,Emmings E,Iizuka Y,Clark C,Starr T,Abrahante JH,Schütz F,Konecny G,Argenta P,Bazzaro M

Mon Oct 01 00:00:00 EDT 2018

RNA Sequencing of Carboplatin- and Paclitaxel-Resistant Endometrial Cancer Cells Reveals New Stratification Markers and Molecular Targets for Cancer Treatment.

Hormones & cancer

Hellweg R,Mooneyham A,Chang Z,Shetty M,Emmings E,Iizuka Y,Clark C,Starr T,Abrahante JH,Schütz F,Konecny G,Argenta P,Bazzaro M

Mon Oct 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: [F-actin]-monooxygenase MICAL2; ERK2-binding testicular protein 1; flavoprotein oxidoreductase MICAL2; MICAL C-terminal-like protein; MICAL-2; Mical-cL; microtubule associated monoxygenase, calponin and LIM domain containing 2; mMical2; Molecule interacting with CasL protein 2; protein MICAL-2; Protein RSB-11-77; protein-methionine sulfoxide oxidase MICAL2

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Gene Aliases: 5330438E18Rik; 9530064J02; KIAA0750; MICAL-2; MICAL2; MICAL2PV1; MICAL2PV2; MICALCL; mKIAA0750; RGD1311773

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UniProt ID: (Human) Q7Z3A8, (Rat) D4A1F2, (Mouse) Q8BML1

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Entrez Gene ID: (Human) 9645, (Rat) 365352, (Mouse) 320878

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Function(s)

Process(es)

heart looping cytoskeleton organization heart development positive regulation of transcription via serum response element binding sulfur oxidation actin filament depolymerization oxidation-reduction process metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MICAL2 Polyclonal Antibody

MICAL2 Antibody (13965-1-AP) in ICC/IF

Product Details

13965-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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