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MIF Polyclonal Antibody

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Questions & Answers

Questions & Answers

Antibody Testing Data (16)

MIF Antibody in Immunocytochemistry (ICC/IF)

MIF Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

MIF Antibody (20415-1-AP) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MIF antibody (Product # 20415-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MIF Antibody in Immunocytochemistry (ICC/IF)

MIF Antibody in Immunocytochemistry (ICC/IF)

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MIF Antibody in Western Blot (WB)

MIF Antibody in Western Blot (WB)

MIF Antibody in Western Blot (WB)

MIF Antibody in Western Blot (WB)

MIF Antibody in Western Blot (WB)

MIF Antibody in Western Blot (WB)

MIF Antibody in Western Blot (WB)

MIF Antibody in Western Blot (WB)

MIF Antibody in Flow Cytometry (Flow)

MIF Antibody in Immunoprecipitation (IP)

Product Image Gallery

MIF Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using MIF antibody (Product # 20415-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MIF Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed Neuro-2a cells using 20415-1-AP (MIF antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using 20415-1-AP (MIF Antibody) at dilution of 1:200.

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate hyperplasia tissue slide using 20415-1-AP (MIF Antibody) at dilution of 1:200.

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 20415-1-AP ( MIF Antibody) at dilution of 1:200.

MIF Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human testis tissue slide using 20415-1-AP ( MIF Antibody) at dilution of 1:200.

MIF Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 20415-1-AP) MIF antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

MIF Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with (Product # 20415-1-AP) MIF antibody at dilution of 1:1,000 incubated at room temperature for 1.5 hours.

MIF Antibody in Western Blot (WB) — Rat spleen tissue were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MIF Antibody in Western Blot (WB) — HL-60 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MIF Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MIF Antibody in Western Blot (WB) — U-937 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MIF Antibody in Western Blot (WB) — Y79 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MIF Antibody in Western Blot (WB) — Y79 cells were subjected to SDS PAGE followed by western blot with 20415-1-AP (MIF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MIF Antibody in Flow Cytometry (Flow) — 1X10^6 THP-1 cells were stained with 0.20 µg MIF antibody (Product # 20415-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

MIF Antibody in Immunoprecipitation (IP) — IP result of anti-MIF (IP:20415-1-AP, 4ug; Detection:20415-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

Product Details

20415-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:50-1:500

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-dependent

-

Immunoprecipitation (IP)

0.5-4.0 µg

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Not applicable (N/A)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MIF Fusion Protein Ag14058 (51-115 aa encoded by BC000447)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GGSSEPCAL CSLHSIGKIG GAQNRSYSKL LCGLLAERLR ISPDRVYINY YDMNAANVGW NNSTFA (51-115 aa encoded by BC000447)

Target Information

MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

International journal of biological sciences

Mao Y,Jiang F,Xu XJ,Zhou LB,Jin R,Zhuang LL,Juan CX,Zhou GP

Wed Feb 15 00:00:00 EST 2023

Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

International journal of biological sciences

Mao Y,Jiang F,Xu XJ,Zhou LB,Jin R,Zhuang LL,Juan CX,Zhou GP

Wed Feb 15 00:00:00 EST 2023

Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

International journal of biological sciences

Mao Y,Jiang F,Xu XJ,Zhou LB,Jin R,Zhuang LL,Juan CX,Zhou GP

Wed Feb 15 00:00:00 EST 2023

Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

International journal of biological sciences

Mao Y,Jiang F,Xu XJ,Zhou LB,Jin R,Zhuang LL,Juan CX,Zhou GP

Wed Feb 15 00:00:00 EST 2023

Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

International journal of biological sciences

Mao Y,Jiang F,Xu XJ,Zhou LB,Jin R,Zhuang LL,Juan CX,Zhou GP

Wed Feb 15 00:00:00 EST 2023

Inhibition of IGF2BP1 attenuates renal injury and inflammation by alleviating m6A modifications and E2F1/MIF pathway.

International journal of biological sciences

Mao Y,Jiang F,Xu XJ,Zhou LB,Jin R,Zhuang LL,Juan CX,Zhou GP

Wed Feb 15 00:00:00 EST 2023

Macrophage migration inhibitory factor (MIF) inhibitor iSO-1 promotes staphylococcal protein A-induced osteogenic differentiation by inhibiting NF-κB signaling pathway.

International immunopharmacology

Shi X,Wu Y,Ni H,Li M,Qi B,Xu Y

Wed Feb 01 00:00:00 EST 2023

Downregulation of macrophage migration inhibitory factor attenuates NLRP3 inflammasome mediated pyroptosis in sepsis-induced AKI.

Cell death discovery

Li T,Sun H,Li Y,Su L,Jiang J,Liu Y,Jiang N,Huang R,Zhang J,Peng Z

Mon Feb 14 00:00:00 EST 2022

TSP50 promotes hepatocyte proliferation and tumour formation by activating glucose-6-phosphate dehydrogenase (G6PD).

Cell proliferation

Zhang X,Gao F,Ai H,Wang S,Song Z,Zheng L,Wang G,Sun Y,Bao Y

Thu Apr 01 00:00:00 EDT 2021

Proteomic analysis of human periodontal ligament cells under hypoxia.

Proteome science

Li Q,Luo T,Lu W,Yi X,Zhao Z,Liu J

Sun Apr 10 00:00:00 EDT 2022

Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.

Molecular carcinogenesis

Wang SS,Zheng M,Pang X,Zhang M,Yu XH,Wu JB,Gao XL,Wu JS,Yang X,Tang YJ,Tang YL,Liang XH

Tue Oct 01 00:00:00 EDT 2019

Macrophage migration inhibitory factor promotes the invasion and metastasis of oral squamous cell carcinoma through matrix metalloprotein-2/9.

Molecular carcinogenesis

Wang SS,Zheng M,Pang X,Zhang M,Yu XH,Wu JB,Gao XL,Wu JS,Yang X,Tang YJ,Tang YL,Liang XH

Tue Oct 01 00:00:00 EDT 2019

Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.

Cancer letters

Liu RM,Sun DN,Jiao YL,Wang P,Zhang J,Wang M,Ma J,Sun M,Gu BL,Chen P,Liu K,Ma H,Gao SG,Ma YF,Qi YJ

Mon Jan 01 00:00:00 EST 2018

Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.

Cancer letters

Liu RM,Sun DN,Jiao YL,Wang P,Zhang J,Wang M,Ma J,Sun M,Gu BL,Chen P,Liu K,Ma H,Gao SG,Ma YF,Qi YJ

Mon Jan 01 00:00:00 EST 2018

Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.

Cancer letters

Liu RM,Sun DN,Jiao YL,Wang P,Zhang J,Wang M,Ma J,Sun M,Gu BL,Chen P,Liu K,Ma H,Gao SG,Ma YF,Qi YJ

Mon Jan 01 00:00:00 EST 2018

Macrophage migration inhibitory factor promotes tumor aggressiveness of esophageal squamous cell carcinoma via activation of Akt and inactivation of GSK3β.

Cancer letters

Liu RM,Sun DN,Jiao YL,Wang P,Zhang J,Wang M,Ma J,Sun M,Gu BL,Chen P,Liu K,Ma H,Gao SG,Ma YF,Qi YJ

Mon Jan 01 00:00:00 EST 2018

Macrophage migration inhibitory factor promotes vasculogenic mimicry formation induced by hypoxia via CXCR4/AKT/EMT pathway in human glioblastoma cells.

Oncotarget

Guo X,Xu S,Gao X,Wang J,Xue H,Chen Z,Zhang J,Guo X,Qian M,Qiu W,Li G

Fri Oct 06 00:00:00 EDT 2017

Macrophage migration inhibitory factor promotes vasculogenic mimicry formation induced by hypoxia via CXCR4/AKT/EMT pathway in human glioblastoma cells.

Oncotarget

Guo X,Xu S,Gao X,Wang J,Xue H,Chen Z,Zhang J,Guo X,Qian M,Qiu W,Li G

Fri Oct 06 00:00:00 EDT 2017

A novel strategy of profiling the mechanism of herbal medicines by combining network pharmacology with plasma concentration determination and affinity constant measurement.

Molecular bioSystems

Chen L,Lv D,Wang D,Chen X,Zhu Z,Cao Y,Chai Y

Tue Oct 18 00:00:00 EDT 2016

A novel strategy of profiling the mechanism of herbal medicines by combining network pharmacology with plasma concentration determination and affinity constant measurement.

Molecular bioSystems

Chen L,Lv D,Wang D,Chen X,Zhu Z,Cao Y,Chai Y

Tue Oct 18 00:00:00 EDT 2016

Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.

BMC microbiology

Li S,Li X,Wang Y,Yang J,Chen Z,Shan S

Fri Feb 07 00:00:00 EST 2014

Global secretome characterization of A549 human alveolar epithelial carcinoma cells during Mycoplasma pneumoniae infection.

BMC microbiology

Li S,Li X,Wang Y,Yang J,Chen Z,Shan S

Fri Feb 07 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Delayed early response protein 6; DER6; GIF; Glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase

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Gene Aliases: GIF; GLIF; MIF; MMIF

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UniProt ID: (Human) P14174, (Mouse) P34884, (Rat) P30904

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Entrez Gene ID: (Human) 4282, (Mouse) 17319, (Rat) 81683

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dopachrome isomerase activity receptor binding cytokine activity cytokine receptor binding protein binding chemoattractant activity phenylpyruvate tautomerase activity isomerase activity protein complex binding decarboxylase

Process(es)

prostaglandin biosynthetic process negative regulation of mature B cell apoptotic process inflammatory response cell surface receptor signaling pathway cell aging cell proliferation negative regulation of gene expression positive regulation of protein kinase A signaling carboxylic acid metabolic process DNA damage response, signal transduction by p53 class mediator positive regulation of B cell proliferation positive regulation of lipopolysaccharide-mediated signaling pathway negative regulation of cellular protein metabolic process negative regulation of myeloid cell apoptotic process positive regulation of peptidyl-serine phosphorylation positive regulation of phosphorylation regulation of macrophage activation negative regulation of apoptotic process positive regulation of MAP kinase activity negative regulation of DNA damage response, signal transduction by p53 class mediator innate immune response positive regulation of fibroblast proliferation positive regulation of cytokine secretion positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration positive chemotaxis positive regulation of prostaglandin secretion involved in immune response positive regulation of myeloid leukocyte cytokine production involved in immune response protein homotrimerization positive regulation of ERK1 and ERK2 cascade negative regulation of cell cycle arrest positive regulation of arachidonic acid secretion negative regulation of cell aging negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of protein phosphorylation brain renin-angiotensin system immune system process positive regulation of acute inflammatory response positive regulation of adaptive immune response spermatogenesis positive regulation of smooth muscle cell migration regulation of cell proliferation drinking behavior positive regulation of potassium ion transport positive regulation of glycolytic process positive regulation of glucose import positive regulation of axon regeneration positive regulation of immune response hair follicle development brain development aging metabolic process response to heat response to mechanical stimulus response to hormone response to inorganic substance response to estradiol response to lipopolysaccharide response to progesterone response to menaquinone response to insulin response to vitamin E wound healing response to drug skin development response to glucocorticoid cellular response to hydrogen peroxide cellular response to hypoxia

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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