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MIF Antibody (PA5-142149) in WB
Western Blot analysis of MIF in Daudi lysate (35 µg protein in RIPA buffer) using MIF Polyclonal Antibody, Biotin (Product # PA5-142149) at 0.2 µg/mL incubated for 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Product Details
PA5-142149
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptide with sequence C-NAANVGWNNSTFA.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2932807
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:128,000.
Target Information
MIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GIF; Glycosylation-inhibiting factor; HGNC:7097; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MGC107654; MIF; Phenylpyruvate tautomerase
Gene Aliases: GIF; GLIF; MIF; MMIF
UniProt ID: (Human) P14174
Entrez Gene ID: (Human) 4282
prostaglandin biosynthetic process
negative regulation of mature B cell apoptotic process
inflammatory response
cell surface receptor signaling pathway
cell aging
cell proliferation
negative regulation of gene expression
positive regulation of protein kinase A signaling
carboxylic acid metabolic process
DNA damage response, signal transduction by p53 class mediator
positive regulation of B cell proliferation
positive regulation of lipopolysaccharide-mediated signaling pathway
negative regulation of cellular protein metabolic process
negative regulation of myeloid cell apoptotic process
positive regulation of peptidyl-serine phosphorylation
positive regulation of phosphorylation
regulation of macrophage activation
negative regulation of apoptotic process
positive regulation of MAP kinase activity
negative regulation of DNA damage response, signal transduction by p53 class mediator
innate immune response
positive regulation of fibroblast proliferation
positive regulation of cytokine secretion
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
positive chemotaxis
positive regulation of prostaglandin secretion involved in immune response
positive regulation of myeloid leukocyte cytokine production involved in immune response
protein homotrimerization
positive regulation of ERK1 and ERK2 cascade
negative regulation of cell cycle arrest
positive regulation of arachidonic acid secretion
negative regulation of cell aging
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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