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MIP-1 delta Antibody (500-P123GBT-25UG) in ELISA
Sandwich ELISA: To detect Human MIP-5 (CCL15) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MIP-1 delta Polyclonal Antibody, Biotin (Product # 500-P123GBT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MIP-1 delta Polyclonal Antibody (Product # 500-P123G-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-5 (CCL15).
Product Details
500-P123GBT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 10.1 kDa Recombinant Human MIP-5 (CCL15)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929345
Product Specific Information
AA Sequence of recombinant protein: QFTNDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI.
Preparation: Produced from sera of goats immunized with highly pure Recombinant Human MIP-5 (CCL15). Anti-Human MIP-5 (CCL15)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human MIP-5 (CCL15) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIP-5 (CCL15) (500-P123G) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-5 (CCL15).
Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hMIP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P123GBT-1MG will be provided as 2 x 500 µg
Target Information
This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; leukotactin 1; Leukotactin-1; LKN-1; Macrophage inflammatory protein 5; MIP-1 delta; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; small inducible cytokine subfamily A (Cys-Cys), member 15; Small-inducible cytokine A15
Gene Aliases: CCL15; HCC-2; HMRP-2B; LKN-1; LKN1; MIP-1 delta; MIP-1D; MIP-5; MIP5; MRP-2B; NCC-3; NCC3; SCYA15; SCYL3; SY15
UniProt ID: (Human) Q16663
Entrez Gene ID: (Human) 6359
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
positive chemotaxis
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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