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Invitrogen
MIP-1 delta Polyclonal Antibody
Advanced Verification
This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
MIP-1 delta Antibody (PA5-46965) in Neu
Neutralization of MIP-1 delta in BaF3 mouse pro‚B cell line transfected with human CCR1. Samples were incubated in MIP-1 delta polyclonal antibody (Product # PA5-46965). Recombinant Human CCL15/MIP‚1δ chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL15/MIP‚1δ (15 ng/mL) is neutralized (green line) by increasing concentrations of Goa... View More
Product Details
PA5-46965
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant human CCL15/MIP-1delta Ser46-Ile113
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607681
Product Specific Information
In direct ELISAs and Western blots, a preference for the 68 aa residue isoform over the 92 aa residue isoform of human CCL15/LKN-1 is shown. This antibody neutralizes the biological activity of the 68 aa isoform of recombinant human (rh) CCL15/LKN-1. This antibody will also neutralize the biological activity of the 92 aa residue isoform of rhCCL15/LKN-1 using a 2-fold higher Ig concentration.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
This gene is located in a cluster of similar genes in the same region of chromosome 17. These genes encode CC cytokines, which are secreted proteins characterized by two adjacent cysteines. The product of this gene is chemotactic for T cells and monocytes, and acts through C-C chemokine receptor type 1 (CCR1). The proprotein is further processed into numerous smaller functional peptides. Naturally-occurring readthrough transcripts occur from this gene into the downstream gene, CCL14 (chemokine (C-C motif) ligand 14).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine 15; chemokine (C-C motif) ligand 15; Chemokine CC-2; HCC-2; HCC-2Lkn-1; leukotactin 1; Leukotactin-1; LKN-1; Macrophage inflammatory protein 5; MIP-1 delta; MIP-5; MIP-5MIP-1d; Mrp-2b; NCC-3; NCC-3MIP-1D; NCC3MRP-2B; new CC chemokine 3; SCYA15member 15; small inducible cytokine subfamily A (Cys-Cys), member 15; Small-inducible cytokine A15
Gene Aliases: CCL15; HCC-2; HMRP-2B; LKN-1; LKN1; MIP-1 delta; MIP-1D; MIP-5; MIP5; MRP-2B; NCC-3; NCC3; SCYA15; SCYL3; SY15
UniProt ID: (Human) Q16663
Entrez Gene ID: (Human) 6359
monocyte chemotaxis
cellular calcium ion homeostasis
chemotaxis
inflammatory response
signal transduction
G-protein coupled receptor signaling pathway
cell-cell signaling
neutrophil chemotaxis
positive regulation of GTPase activity
lymphocyte chemotaxis
positive regulation of inflammatory response
positive chemotaxis
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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