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MIP-1 gamma Antibody (500-P117BT-25UG) in ELISA
Sandwich ELISA: To detect Murine MIP-1gamma (CCL9/10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of MIP-1 gamma Polyclonal Antibody, Biotin (Product # 500-P117BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech MIP-1 gamma Polyclonal Antibody (Product # 500-P117-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-1gamma (CCL9/10).
Product Details
500-P117BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine MIP-1gamma (CCL9/10)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929298
Product Specific Information
AA Sequence of recombinant protein: QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine MIP-1gamma (CCL9/10). Anti-Murine MIP-1gamma (CCL9/10)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Murine MIP-1gamma (CCL9/10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine MIP-1gamma (CCL9/10) (500-P117) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine MIP-1gamma (CCL9/10).
Western Blot: To detect Murine MIP-1gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine MIP-1gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P117BT-1MG will be provided as 2 x 500 µg
Target Information
MIP-1gamma is a CC chemokine found in murine blood and a wide variety of murine tissues, with no known human homolog. MIP-1gamma signals through the CCR1 receptor. MIP-1gamma chemoattracts neutrophils, and also inhibits colony formation of bone marrow myeloid immature progenitors. MIP-1gamma contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C-C motif chemokine 9; CCF18; Macrophage inflammatory protein 1-gamma; Macrophage inflammatory protein-related protein 2; MIP-1 gamma; MIP-1-gamma; MRP-2; RP23-320E6.3; small inducible cytokine A10; small inducible cytokine A9; Small-inducible cytokine A9
Gene Aliases: CCF18; Ccl9; MRP-2; Mrp2; Scya10; Scya9
UniProt ID: (Mouse) P51670
Entrez Gene ID: (Mouse) 20308
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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