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MIP-3 beta Antibody (500-P95B-1MG) in ELISA
Sandwich ELISA: To detect Human MIP-3beta (CCL19) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of MIP-3 beta Polyclonal Antibody (Product # 500-P95B-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech MIP-3 beta Polyclonal Antibody, Biotin (Product # 500-P95BBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3beta (CCL19).
Product Details
500-P95B-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human MIP-3beta (CCL19)
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930882
Product Specific Information
AA Sequence of recombinant protein: GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3beta (CCL19). Anti-Human MIP-3beta (CCL19)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-3beta (CCL19) matrix.
Sandwich ELISA: To detect Human MIP-3beta (CCL19) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-3beta (CCL19) (500-P95BBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3beta (CCL19).
Western Blot: To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta chemokine exodus-3; Beta-chemokine exodus-3; C-C motif chemokine; C-C motif chemokine 19; CC chemokine ligand 19; chemokine (C-C motif) ligand 19; CK beta-11; EBI1 ligand chemokine; EBI1-ligand chemokine; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; M-MIP-3-beta; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MIP-3-beta; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19
Gene Aliases: CCL19; CKb11; ELC; MIP-3b; MIP3B; SCYA19
UniProt ID: (Human) Q99731
Entrez Gene ID: (Human) 6363
establishment of T cell polarity
immunological synapse formation
dendritic cell chemotaxis
myeloid dendritic cell chemotaxis
monocyte chemotaxis
positive regulation of dendritic cell antigen processing and presentation
cellular calcium ion homeostasis
inflammatory response
immune response
cell communication
G-protein coupled receptor signaling pathway
activation of JUN kinase activity
response to virus
positive regulation of glycoprotein biosynthetic process
T cell costimulation
positive regulation of interleukin-12 production
positive regulation of tumor necrosis factor production
response to prostaglandin E
positive regulation of T cell proliferation
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of T-helper 1 cell differentiation
positive regulation of endocytosis
positive regulation of protein kinase activity
positive regulation of JNK cascade
lymphocyte chemotaxis
positive regulation of receptor-mediated endocytosis
cell maturation
positive regulation of interleukin-1 beta secretion
positive regulation of chemotaxis
release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
regulation of cell projection assembly
chemokine-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
response to nitric oxide
interleukin-12 secretion
positive regulation of neutrophil chemotaxis
mature conventional dendritic cell differentiation
cellular response to virus
negative regulation of leukocyte apoptotic process
positive regulation of cell motility
positive regulation of dendritic cell dendrite assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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