Invitrogen

MIP-3 beta Polyclonal Antibody, Biotin, PeproTech®

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View all (23) CCL19 antibodies
Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

500-P95BBT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived, 8.8 kDa Recombinant Human MIP-3beta (CCL19)
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930885

Product Specific Information

AA Sequence of recombinant protein: GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3beta (CCL19). Anti-Human MIP-3beta (CCL19)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect Human MIP-3beta by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MIP-3beta (500-P95B) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3beta.

Western Blot: To detect Human MIP-3beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human MIP-3beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P95BBT-1MG will be provided as 2 x 500 µg

Target Information

CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta chemokine exodus-3; Beta-chemokine exodus-3; C-C motif chemokine; C-C motif chemokine 19; CC chemokine ligand 19; chemokine (C-C motif) ligand 19; CK beta-11; EBI1 ligand chemokine; EBI1-ligand chemokine; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; M-MIP-3-beta; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MIP-3-beta; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

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Gene Aliases: CCL19; CKb11; ELC; MIP-3b; MIP3B; SCYA19

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UniProt ID: (Human) Q99731

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Entrez Gene ID: (Human) 6363

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Function(s)
chemokine activity CCR7 chemokine receptor binding CCR10 chemokine receptor binding chemokine receptor binding CCR chemokine receptor binding cytokine intercellular signal molecule
Process(es)
establishment of T cell polarity immunological synapse formation dendritic cell chemotaxis myeloid dendritic cell chemotaxis monocyte chemotaxis positive regulation of dendritic cell antigen processing and presentation cellular calcium ion homeostasis inflammatory response immune response cell communication G-protein coupled receptor signaling pathway activation of JUN kinase activity response to virus positive regulation of glycoprotein biosynthetic process T cell costimulation positive regulation of interleukin-12 production positive regulation of tumor necrosis factor production response to prostaglandin E positive regulation of T cell proliferation positive regulation of NF-kappaB import into nucleus positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity positive regulation of T-helper 1 cell differentiation positive regulation of endocytosis positive regulation of protein kinase activity positive regulation of JNK cascade lymphocyte chemotaxis positive regulation of receptor-mediated endocytosis cell maturation positive regulation of interleukin-1 beta secretion positive regulation of chemotaxis release of sequestered calcium ion into cytosol positive regulation of protein kinase B signaling regulation of cell projection assembly chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor response to nitric oxide interleukin-12 secretion positive regulation of neutrophil chemotaxis mature conventional dendritic cell differentiation cellular response to virus negative regulation of leukocyte apoptotic process positive regulation of cell motility positive regulation of dendritic cell dendrite assembly
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