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MIP-3 beta Antibody (MA5-23845) in Neu
Neutralization of MIP-3 beta in BaF3 mouse pro‚B cell line transfected with human CCR7. Samples were incubated in MIP-3 beta monoclonal antibody (Product # MA5-23845). Recombinant Mouse CCL19/MIP‚3β chemoattracts the BaF3 mouse pro‚B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL19/MIP‚3β (50 ng/mL) is neutralized (green line) by increasing concentrationsof Rat An... View More
Product Details
MA5-23845
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2A
Class
Monoclonal
Type
Antibody
Clone
87102
Immunogen
E. coli-derived recombinant mouse CCL19/MIP-3 beta Gly26-Val107-Leu-Glu (Ser108LeuGlu)
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2610117
Product Specific Information
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
CCL19, also known as MIP-3 beta (Macrophage Inflammatory Protein 3 beta), is a member of the CC- subfamily of chemokines. It is most closely related to CCL21, with which it shares 32% amino acid sequence homology. CCL19 and CCL21 are expressed mainly by stromal cells in the T cell-rich zones of lymph nodes. They are critical mediators of the homeostatic trafficking of naive T cells and activated dendritic cells into the secondary lymphoid organs. CCL19 and CCL21 also play a role in T cell priming and activation, as well as the recruitment of lymphocytes to inflamed tissue. Both proteins signal via the G protein-coupled receptor, CCR7, which is expressed on T cells and mature dendritic cells. Although CCL19 and CCL21 both exhibit the same affinity for CCR7, only the binding of CCL19 results in the desensitization and internalization of the receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-C motif chemokine; C-C motif chemokine 19; chemokine CCL19; EBI-1 ligand chemokine; EBI1 ligand chemokine; EBI1-ligand chemokine; EBV-induced molecule 1 ligand chemokine; Epstein-Barr virus-induced molecule 1 ligand chemokine; M-MIP-3-beta; small inducible cytokine A19; Small-inducible cytokine A19
Gene Aliases: Ccl19; CKb11; ELC; exodus-3; MIP3B; Scya19
UniProt ID: (Mouse) O70460
Entrez Gene ID: (Mouse) 24047
establishment of T cell polarity
dendritic cell chemotaxis
myeloid dendritic cell chemotaxis
response to prostaglandin E
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of phosphatidylinositol 3-kinase activity
positive regulation of protein kinase activity
positive regulation of JNK cascade
release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
positive regulation of ERK1 and ERK2 cascade
response to nitric oxide
positive regulation of neutrophil chemotaxis
negative regulation of leukocyte apoptotic process
positive regulation of cell motility
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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