Invitrogen

IL-12 p70 Monoclonal Antibody (C17-8)

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Details

MM121

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 10 publications 10 publications

Neutralization (Neu)

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

C17-8

Immunogen

Recombinant mouse IL-12(p70) (CHO cell-derived)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223526

Product Specific Information

MM121 targets IL-12 in ELISA and WB applications and shows reactivity with mouse samples.

The MM121 immunogen is recombinant mouse IL-12(p70) (CHO cell-derived).

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nanomaterials-Mediated Co-Stimulation of Toll-Like Receptors and CD40 for Antitumor Immunity.

Advanced materials (Deerfield Beach, Fla.)

Yan J,Zhang Y,Du S,Hou X,Li W,Zeng C,Zhang C,Cheng J,Deng B,McComb DW,Zhao W,Xue Y,Kang DD,Cheng X,Dong Y

MM121 was used in Enzyme-linked immunosorbent assay to develop an immunotherapy regimen that enables co-stimulation of TLR7/8- and CD40-mediated pathways.

Tue Nov 01 00:00:00 EDT 2022

Lymphocytic Microparticles Modulate Angiogenic Properties of Macrophages in Laser-induced Choroidal Neovascularization.

Scientific reports

Tahiri H,Omri S,Yang C,Duhamel F,Samarani S,Ahmad A,Vezina M,Bussières M,Vaucher E,Sapieha P,Hickson G,Hammamji K,Lapointe R,Rodier F,Tremblay S,Royal I,Cailhier JF,Chemtob S,Hardy P

MM121 was used in flow cytometry to study the ability of lymphocyte-derived microparticles to modulate the gene expression pattern of angiogenesis-related factors in macrophages

Tue Nov 22 00:00:00 EST 2016

A polymorphism in the coding region of Il12b promotes IL-12p70 and IL-23 heterodimer formation.

Journal of immunology (Baltimore, Md. : 1950)

Zwiers A,Fuss IJ,Seegers D,Konijn T,Garcia-Vallejo JJ,Samsom JN,Strober W,Kraal G,Bouma G

MM121 was used in western blot to investigate the effect of a polymorphism in the coding region of Il12b for interleukin-12p70 and interleukin-23 heterodimer formation

Tue Mar 15 00:00:00 EDT 2011

Ethanol consumption modifies dendritic cell antigen presentation in mice.

Alcoholism, clinical and experimental research

Heinz R,Waltenbaugh C

MM121 was used in ELISA to assess the influence of alcohol consumption on antigen-presenting cell populations and cytokine production in type 1 cell-mediated adaptive immune responses

Mon Oct 01 00:00:00 EDT 2007

Heligmosomoides polygyrus promotes regulatory T-cell cytokine production in the murine normal distal intestine.

Infection and immunity

Setiawan T,Metwali A,Blum AM,Ince MN,Urban JF,Elliott DE,Weinstock JV

MM121 was used in ELISA to investigate the effect of exposure to Heligmosomoides polygyrus on cytokine pathways in murine normal distal intestine

Sat Sep 01 00:00:00 EDT 2007

Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection.

Cancer research

Guiducci C,Vicari AP,Sangaletti S,Trinchieri G,Colombo MP

MM121 was used in ELISA to describe the mechanisms by which tumor-infiltrating macrophages and dendritic cells can be redirected to become potent effectors and activators of the innate and adaptive immunity

Fri Apr 15 00:00:00 EDT 2005

The influence of collagenase treatment on the production of TNF-alpha, IL-6 and IL-10 by testicular macrophages.

Journal of immunological methods

Bryniarski K,Szczepanik M,Ptak M,Ptak W

MM121 was used in ELISA to study the effect of collagenase treatment on cytokine production by testicular macrophages

Wed Jun 01 00:00:00 EDT 2005

Modulation of testicular macrophage activity by collagenase.

Folia histochemica et cytobiologica

Bryniarski K,Szczepanik M,Ptak M,Ptak W

MM121 was used in ELISA to investigate the regulation of cytokines and the expression of ROI in testicular macrophages

Tue Aug 09 00:00:00 EDT 2005

Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination.

Experimental & molecular medicine

Kim A,Noh YW,Kim KD,Jang YS,Choe YK,Lim JS

MM121 was used in blocking/activating experiment to investigate the effect of dendritic cell vaccination for the prevention of tumor migration

Sun Oct 31 00:00:00 EDT 2004

Heligmosomoides polygyrus inhibits established colitis in IL-10-deficient mice.

European journal of immunology

Elliott DE,Setiawan T,Metwali A,Blum A,Urban JF,Weinstock JV

MM121 was used in ELISA to investigate the effect of Heligmosomoides polygyrus on colitis

Fri Oct 01 00:00:00 EDT 2004

In vivo compartmentalization of functionally distinct, rapidly responsive antigen-specific T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice.

Infection and immunity

Kirby AC,Sundquist M,Wick MJ

MM121 was used in blocking or activating experiment to characterize specific memory T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice

Mon Nov 01 00:00:00 EST 2004

NF-kappa B is required within the innate immune system to inhibit microflora-induced colitis and expression of IL-12 p40.

Journal of immunology (Baltimore, Md. : 1950)

Tomczak MF,Erdman SE,Poutahidis T,Rogers AB,Holcombe H,Plank B,Fox JG,Horwitz BH

MM121 was used in ELISA to study the role of NF-kappa B and the innate immune system in inhibiting microflora-induced colitis

Fri Aug 01 00:00:00 EDT 2003

Modulation of macrophage activity by proteolytic enzymes. Differential regulation of IL-6 and reactive oxygen intermediates (ROIs) synthesis as a possible homeostatic mechanism in the control of inflammation.

Inflammation

Bryniarski K,Maresz K,Szczepanik M,Ptak M,Ptak W

MM121 was used in ELISA to investigate the effect of collagenase and trypsin for the regulation of macrophage function

Mon Dec 01 00:00:00 EST 2003

Quantities of interleukin-12p40 in mature CD8alpha negative dendritic cells correlate with strength of TCR signal and determine Th cell development.

Microbiology and immunology

Fujimoto N,Ishida H,Nakamura I,Ogasawara K,Itoh Y

MM121 was used in ELISA and flow cytometry to report that the strength of TCR signaling directs Th cell development by regulating CD40L expression on T cells which determines production of IL-12p40 from DC via CD40.

Fri Mar 05 00:00:00 EST 2004

Corticosteroids enhance the capacity of macrophages to induce Th2 cytokine synthesis in CD4+ lymphocytes by inhibiting IL-12 production.

Journal of immunology (Baltimore, Md. : 1950)

DeKruyff RH,Fang Y,Umetsu DT

MM121 was used in ELISA to investigate the changes of IL-12 expression modulated by corticosteroids in CD4+ T cells

Sun Mar 01 00:00:00 EST 1998

CD40 ligation prevents neonatal induction of transplantation tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Flamand V,Donckier V,Demoor FX,Le Moine A,Matthys P,Vanderhaeghen ML,Tagawa Y,Iwakura Y,Billiau A,Abramowicz D,Goldman M

MM121 was used in blocking/activating experiment to study the effect of CD40 ligation on neonatal transplantation tolerance

Fri May 15 00:00:00 EDT 1998

Interleukin-12 is produced by dendritic cells and mediates T helper 1 development as well as interferon-gamma production by T helper 1 cells.

European journal of immunology

Heufler C,Koch F,Stanzl U,Topar G,Wysocka M,Trinchieri G,Enk A,Steinman RM,Romani N,Schuler G

MM121 was used in blocking/activating experiment to investigate the role of interleukin 12 in Th1 cell function

Fri Mar 01 00:00:00 EST 1996

Characterization of early IL-12, IFN-alphabeta, and TNF effects on antiviral state and NK cell responses during murine cytomegalovirus infection.

Journal of immunology (Baltimore, Md. : 1950)

Orange JS,Biron CA

MM121 was used in blocking/activating experiment to investigate the importance of tumor necrosis factor, interleukin 12 and interferon alpha beta in immune responses against cytomegalovirus infection

Sat Jun 15 00:00:00 EDT 1996

Bioinformatics

Protein Aliases: 20C2; CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL12A/B; IL12AB; ILN; Interleukin; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; MGC151228; MGC151232

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; Il12a; Il12b; Il12p40; Ll12a; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160

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Function(s)

transmembrane signal receptor

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-12 p70 Monoclonal Antibody (C17-8)

Product Details

MM121

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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