Invitrogen

IL-5 Monoclonal Antibody (TRFK5)

Product Details

MM550C

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Flow Cytometry (Flow)

View 3 publications 3 publications

ELISA (ELISA)

Assay-dependent

View 8 publications 8 publications

Neutralization (Neu)

View 2 publications 2 publications

Inhibition Assays (IA)

Assay-dependent

Blocking Assay (BLOCK)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

TRFK5

Immunogen

Recombinant mouse IL-5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223533

Product Specific Information

MM550C targets IL-5 in ELISA, IA, and WB applications and shows reactivity with mouse samples.

The MM550C immunogen is recombinant mouse IL-5.

MM550C detects IL-5 which has a predicted molecular weight of approximately 13 kDa.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product is a Low Endotoxin formulation.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A mechanism for the induction of type 2 immune responses by a protease allergen in the genital tract.

Proceedings of the National Academy of Sciences of the United States of America

Oh JE,Oh DS,Jung HE,Lee HK

MM550C was used in blocking or activating experiment to propose that type 2 immunity is induced by a unique mechanism in the genital tract

Tue Feb 14 00:00:00 EST 2017

Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody.

Journal of immunological methods

Liao K,Meyer E,Lee TN,Loercher A,Sikkema D

MM550C was used in blocking or activating experiment to compare solid-phase immunodepletion of IL-5 with an in-solution IL-5 immunocompetition

Wed Feb 01 00:00:00 EST 2017

IL-25-responsive, lineage-negative KLRG1(hi) cells are multipotential 'inflammatory' type 2 innate lymphoid cells.

Nature immunology

Huang Y,Guo L,Qiu J,Chen X,Hu-Li J,Siebenlist U,Williamson PR,Urban JF,Paul WE

MM550C was used in Flow Cytometry to characterise the existence of an inflammatory ILC2 population responsive to interleukin 25 that complements IL-33-responsive natural ILC2 cells.

Sun Feb 01 00:00:00 EST 2015

Role of arginase 1 from myeloid cells in th2-dominated lung inflammation.

PloS one

Barron L,Smith AM,El Kasmi KC,Qualls JE,Huang X,Cheever A,Borthwick LA,Wilson MS,Murray PJ,Wynn TA

MM550C was used in flow cytometry to determine the contribution of Arg1 to lung inflammation and pathophysiology

Mon Dec 09 00:00:00 EST 2013

Influence of mucosal adjuvants on antigen passage and CD4+ T cell activation during the primary response to airborne allergen.

Journal of immunology (Baltimore, Md. : 1950)

Wikstrom ME,Batanero E,Smith M,Thomas JA,von Garnier C,Holt PG,Stumbles PA

MM550C was used in flow cytometry to study the effects of Escherichia coli LPS and cholera toxin on antigen passage and T cell activation following nasal administration OVA

Sat Jul 15 00:00:00 EDT 2006

Chronic intestinal nematode infection induces Stat6-independent interleukin-5 production and causes eosinophilic inflammatory responses in mice.

Immunology

Sakamoto Y,Hiromatsu K,Ishiwata K,Inagaki-Ohara K,Ikeda T,Nakamura-Uchiyama F,Nawa Y

MM550C was used in ELISA to investigate the role of STAT6 for the immune response induced by Nippostrongylus brasiliensis

Sun Aug 01 00:00:00 EDT 2004

Effects of birch pollen and traffic particulate matter on Th2 cytokines, immunoglobulin E levels and bronchial hyper-responsiveness in mice.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Fernvik E,Peltre G,Sénéchal H,Vargaftig BB

MM550C was used in ELISA to study the role of pollen and traffic particulate matter in the initiation and persistence of experimental allergic inflammation

Mon Apr 01 00:00:00 EST 2002

Murine strain differences in airway inflammation caused by diesel exhaust particles.

The European respiratory journal

Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M

MM550C was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants

Sun Feb 01 00:00:00 EST 1998

Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.

Immunology

Bordmann G,Rudin W,Favre N

MM550C was used in ELISA to investigate the effect of phosphatidylcholine immunization of mice for the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections

Fri May 01 00:00:00 EDT 1998

Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.

The Journal of pharmacology and experimental therapeutics

Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M

MM550C was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression

Sat Aug 01 00:00:00 EDT 1998

Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.

Toxicological sciences : an official journal of the Society of Toxicology

Ichinose T,Takano H,Miyabara Y,Sagai M

MM550C was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system

Wed Jul 01 00:00:00 EDT 1998

Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.

American journal of respiratory and critical care medicine

Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M

MM550C was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression

Tue Jul 01 00:00:00 EDT 1997

Delineation of IL-5 domains predicted to engage the IL-5 receptor complex.

Journal of immunology (Baltimore, Md. : 1950)

Dickason RR,Huston MM,Huston DP

MM550C was used in blocking/activating experiment to study the mechanism for the effect of interleukin 5

Thu Feb 01 00:00:00 EST 1996

Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.

Journal of immunology (Baltimore, Md. : 1950)

Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E

MM550C was used in blocking/activating experiment to investigate the effect of CD4 antibodies on Th1 differentiation

Mon Jul 15 00:00:00 EDT 1996

Synthesis of T helper 2-type cytokines at the maternal-fetal interface.

Journal of immunology (Baltimore, Md. : 1950)

Lin H,Mosmann TR,Guilbert L,Tuntipopipat S,Wegmann TG

MM550C was used in ELISA to investigate the expression of Th2 cytokines at the maternal-fetal interface

Mon Nov 01 00:00:00 EST 1993

Bioinformatics

Protein Aliases: B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF

View more View less

Gene Aliases: Il-5; Il5

View more View less

UniProt ID: (Mouse) P04401

View more View less

Entrez Gene ID: (Mouse) 16191

View more View less

Function(s)

cytokine activity interleukin-5 receptor binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-5 Monoclonal Antibody (TRFK5)

Product Details

MM550C

Western Blot (WB)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Inhibition Assays (IA)

Blocking Assay (BLOCK)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!