Invitrogen

IL-5 Monoclonal Antibody (TRFK4)

Product Details

MM550D

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 9 publications 9 publications

Neutralization (Neu)

View 2 publications 2 publications

Inhibition Assays (IA)

Assay-dependent

Blocking Assay (BLOCK)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TRFK4

Immunogen

Recombinant mouse IL-5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223534

Product Specific Information

MM550D targets IL-5 in ELISA, IA, and WB applications and shows reactivity with mouse samples.

The MM550D immunogen is recombinant mouse IL-5.

MM550D detects IL-5 which has a predicted molecular weight of approximately 13 kDa.

The MM550D mouse IL5 antibody (clone TRFK4) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody MM550CB (biotinylated conjugate of clone TRFK5). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Antibody MM550D (clone TRFK4) and biotinylated antibody MM550CB (clone TRFK5) have successfully been used in combination with recombinant mouse IL5 protein SMIL5 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

This product is a Low Endotoxin formulation.

This product has been tested for endotoxins by limulus amoebocyte lysate (LAL) assay and contains an endotoxin concentration of less than or equal to 10 endotoxin units per milligram (EU/mg).

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Group 2 Innate Lymphoid Cells Promote an Early Antibody Response to a Respiratory Antigen in Mice.

Journal of immunology (Baltimore, Md. : 1950)

Drake LY,Iijima K,Bartemes K,Kita H

MM550D was used in blocking or activating experiment to suggest that lung group 2 innate lymphoid cells promote B cell production of early antibodies to a respiratory antigen even in the absence of T cells

Mon Aug 15 00:00:00 EDT 2016

Intratracheal myriocin enhances allergen-induced Th2 inflammation and airway hyper-responsiveness.

Immunity, inflammation and disease

Edukulla R,Rehn KL,Liu B,McAlees JW,Hershey GK,Wang YH,Lewkowich I,Lindsley AW

MM550D was used in ELISA to discuss the link between sphingolipid regulatory genes and asthma pathogenesis

Thu Sep 01 00:00:00 EDT 2016

Chronic intestinal nematode infection induces Stat6-independent interleukin-5 production and causes eosinophilic inflammatory responses in mice.

Immunology

Sakamoto Y,Hiromatsu K,Ishiwata K,Inagaki-Ohara K,Ikeda T,Nakamura-Uchiyama F,Nawa Y

MM550D was used in ELISA to investigate the role of STAT6 for the immune response induced by Nippostrongylus brasiliensis

Sun Aug 01 00:00:00 EDT 2004

Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.

Journal of immunology (Baltimore, Md. : 1950)

Chen M,Hu KF,Rozell B,Orvell C,Morein B,Liljeström P

MM550D was used in ELISA to study the vaccination against respiratory syncytial virus

Sun Sep 15 00:00:00 EDT 2002

Effects of birch pollen and traffic particulate matter on Th2 cytokines, immunoglobulin E levels and bronchial hyper-responsiveness in mice.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Fernvik E,Peltre G,Sénéchal H,Vargaftig BB

MM550D was used in ELISA to study the role of pollen and traffic particulate matter in the initiation and persistence of experimental allergic inflammation

Mon Apr 01 00:00:00 EST 2002

Murine strain differences in airway inflammation caused by diesel exhaust particles.

The European respiratory journal

Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M

MM550D was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants

Sun Feb 01 00:00:00 EST 1998

Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.

The Journal of pharmacology and experimental therapeutics

Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M

MM550D was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression

Sat Aug 01 00:00:00 EDT 1998

Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.

Toxicological sciences : an official journal of the Society of Toxicology

Ichinose T,Takano H,Miyabara Y,Sagai M

MM550D was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system

Wed Jul 01 00:00:00 EDT 1998

Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.

American journal of respiratory and critical care medicine

Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M

MM550D was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression

Tue Jul 01 00:00:00 EDT 1997

Delineation of IL-5 domains predicted to engage the IL-5 receptor complex.

Journal of immunology (Baltimore, Md. : 1950)

Dickason RR,Huston MM,Huston DP

MM550D was used in blocking/activating experiment to study the mechanism for the effect of interleukin 5

Thu Feb 01 00:00:00 EST 1996

Th1 development of naive CD4+ T cells is inhibited by co-activation with anti-CD4 monoclonal antibodies.

Journal of immunology (Baltimore, Md. : 1950)

Goedert S,Germann T,Hoehn P,Koelsch S,Palm N,Rüde E,Schmitt E

MM550D was used in blocking/activating experiment to investigate the effect of CD4 antibodies on Th1 differentiation

Mon Jul 15 00:00:00 EDT 1996

Synthesis of T helper 2-type cytokines at the maternal-fetal interface.

Journal of immunology (Baltimore, Md. : 1950)

Lin H,Mosmann TR,Guilbert L,Tuntipopipat S,Wegmann TG

MM550D was used in ELISA to investigate the expression of Th2 cytokines at the maternal-fetal interface

Mon Nov 01 00:00:00 EST 1993

Bioinformatics

Protein Aliases: B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF

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Gene Aliases: Il-5; Il5

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UniProt ID: (Mouse) P04401

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Entrez Gene ID: (Mouse) 16191

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Function(s)

cytokine activity interleukin-5 receptor binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-5 Monoclonal Antibody (TRFK4)

Product Details

MM550D

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Inhibition Assays (IA)

Blocking Assay (BLOCK)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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