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MMP12 Antibody (MA5-42836) in WB
Western blot analysis of MMP12 in extracts of various cell lines. Samples were incubated with MMP12 Monoclonal antibody (Product # MA5-42836) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
Product Details
MA5-42836
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1O3A4
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 371-470 of human MMP12 (P39900).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911977
Product Specific Information
Positive test controls include: A-549, BxPC-3, Mouse lung, Mouse brain, Mouse spleen, Rat lung. The target is usually found in the following locations: Secreted, extracellular matrix, extracellular space.
Immunogen sequence: FGFPNFVKKI DAAVFNPRFY RTYFFVDNQY WRYDERRQMM DPGYPKLITK NFQGIGPKID AVFYSKNKYY YFFQGSNQFE YDFLLQRITK TLKSNSWFGC
Target Information
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. 3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3.4.24.65; Macrophage elastase; Macrophage metalloelastase; macrophage-metalloelastase; matrix metallo protease; matrix metallopeptidase 12 (macrophage elastase); matrix metalloproteinase 12; matrix metalloproteinase 12 (macrophage elastase); Matrix metalloproteinase-12; ME; MGC138506; MME; MMP; MMP-12; MMPs
Gene Aliases: AV378681; HME; ME; MME; Mmel; MMP-12; MMP12
UniProt ID: (Human) P39900, (Mouse) P34960, (Rat) Q63341
Entrez Gene ID: (Human) 4321, (Mouse) 17381, (Rat) 117033
proteolysis
response to organic cyclic compound
extracellular matrix disassembly
collagen catabolic process
wound healing, spreading of epidermal cells
response to drug
positive regulation of epithelial cell proliferation involved in wound healing
wound healing
positive regulation of epithelial cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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