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MMP2 Monoclonal Antibody (CA-4001 (CA719E3C)), Biotin

1 Published Figure

21 References

View all (46) MMP2 antibodies

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Antibody Testing Data (1)
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MMP2 Antibody (MA5-13587) in WB

Western blot of MMP-2 (72kDa Collagenase IV) using MMP-2 (72kDa Collagenase IV) Monoclonal Antibody (Product # MA5-13587) on HFL-1+TPA med Cells.

MMP2 Antibody in Immunohistochemistry (IHC)

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Product Details

MA5-13587

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Neutralization (Neu)

View 2 publications 2 publications

Inhibition Assays (IA)

2-4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Chicken, Dog, Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CA-4001 (CA719E3C)

Immunogen

N-terminal peptide APSPIIKFPGD-VAPKTDK of procollagenase IV

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10977368

Product Specific Information

MA5-13587 targets MMP-2 (72kDa Collagenase IV) in IA and WB applications and shows reactivity with Human, mouse, and Rat samples.

The MA5-13587 immunogen is n-terminal peptide APSPIIKFPGD-VAPKTDK of procollagenase IV.

Target Information

MMP (matrix metalloproteinase) are proteolytic enzymes capable of degrading connective tissue components. MMP have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPa and TIMPs play a significant role in regulating angiogenesis. MMP2 is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids, and contains the basic structure of propeptide, catalytic, and hemopexin domains. The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. Functionally, MMP2 is involved in tissue remodeling. Mutations in MMP-2 gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms of MMP-2 have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

α11β1 integrin-mediated MMP-13-dependent collagen lattice contraction by fibroblasts: evidence for integrin-coordinated collagen proteolysis.

Journal of cellular physiology

Barczyk MM,Lu N,Popova SN,Bolstad AI,Gullberg D

MA5-13587 was used in western blot to study the role of mechanical strain generated by alpha11beta1 integrin in MMP-13-mediated fibroblast collagen lattice remodelling

Wed May 01 00:00:00 EDT 2013

Expression of matrix metalloproteinase-12 is correlated with extracapsular spread of tumor from nodes with metastasis in head and neck squamous cell carcinoma.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery

Kim JM,Kim HJ,Koo BS,Rha KS,Yoon YH

MA5-13587 was used in immunohistochemistry to study the relationship between expression of MMP-12 in head and neck squamous cell carcinoma primary tumors and extracapsular spread of lymph node metastases

Fri Mar 01 00:00:00 EST 2013

Matrix metalloproteinase, vitamin A and methylprednisolone effects on experimentally induced amyloid arthropathy.

Biotechnic & histochemistry : official publication of the Biological Stain Commission

Sevimli A,Yağcı A,Bülbül A

MA5-13587 was used in immunohistochemistry to study the effects of vitamin A and methylprednisolone on the expression of MMP-1, -2 and -9 in a chick model of amyloid arthropathy

Thu Aug 01 00:00:00 EDT 2013

Induction of pulmonary fibrosis by cerium oxide nanoparticles.

Toxicology and applied pharmacology

Ma JY,Mercer RR,Barger M,Schwegler-Berry D,Scabilloni J,Ma JK,Castranova V

MA5-13587 was used in immunohistochemistry to study the ability of cerium oxide nanoparticles to induce pulmonary fibrosis in a rat model

Wed Aug 01 00:00:00 EDT 2012

MMP-2 expression in 102 patients with glottic laryngeal cancer.

Mallis A,Teymoortash A,Mastronikolis NS,Werner JA,Papadas TA

MA5-13587 was used in immunohistochemistry to study MMP-2 expression in patients with glottic laryngeal cancer and its potential prognostic value

Wed Feb 01 00:00:00 EST 2012

Gelatinases A and B expression in human colorectal cancer in upper Egypt: a clinicopathological study.

Ultrastructural pathology

Omran OM,Thabet M

MA5-13587 was used in immunohistochemistry to study the immunohistochemical expression of MMP2 and MMP9 in colorectal cancer in an upper Egypt population

Sun Apr 01 00:00:00 EDT 2012

Down-regulation of matrix metalloproteinase-9 by pyrrolidine dithiocarbamate prevented retinal ganglion cell death after transection of optic nerve in rats.

Current eye research

Sun MH,Chen KJ,Tsao YP,Kao LY,Han WH,Lin KK,Pang JH

MA5-13587 was used in western blot to study the effects of pyrrolidine dithiocarbamate on MMP2 and MMP9 expresssion and MMP-mediated apoptosis in retinal ganglion cells following transection of the optic nerve

Tue Nov 01 00:00:00 EDT 2011

The effects of interferon alpha2b on chemically-induced peritoneal fibrosis and on peritoneal tissue MMP-2 and TIMP-2 levels in rats.

The Journal of international medical research

Ucar E,Borazan A,Semerci E,Binici DN,Yaldiz M,Aysal A,Altug E,Kuvandik C,Huzmeli C,Yetim T,Canda S

MA5-13587 was used in immunohistochemistry to characterize effects of alpha2b treatment on fibrosis and metalloproteinase levels

Thu Jun 03 00:00:00 EDT 2010

SK-PC-B70M confers anti-oxidant activity and reduces Abeta levels in the brain of Tg2576 mice.

Brain research

Seo JS,Kim TK,Leem YH,Lee KW,Park SK,Baek IS,Kim KS,Im GJ,Lee SM,Park YH,Han PL

MA5-13587 was used in western blot to study the effect of an oleanolic-glycoside saponin on anti-oxidant activity and Abeta levels in the brain of Tg2576 mice.

Thu Mar 19 00:00:00 EDT 2009

GSK-3-specific inhibitor-supplemented hESC medium prevents the epithelial-mesenchymal transition process and the up-regulation of matrix metalloproteinases in hESCs cultured in feeder-free conditions.

Molecular human reproduction

Ullmann U,Gilles C,De Rycke M,Van de Velde H,Sermon K,Liebaers I

MA5-13587 was used in immunocytochemistry to investigate the effect of an inhibitor against GSK-3 on the epithelial-mesenchymal transition process and the maintenance of th pluripotency of stem cells

Sat Mar 01 00:00:00 EST 2008

Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARgamma Dependent and Independent Mechanisms.

PPAR research

He Q,Pang R,Song X,Chen J,Chen H,Chen B,Hu P,Chen M

MA5-13587 was used in western blot to investigate the effect of rosiglitazone on SGC-7901 gastric cancer cells and its mechanism

Thu Jul 14 00:00:00 EDT 2011

The hepatitis B virus X protein promotes hepatocellular carcinoma metastasis by upregulation of matrix metalloproteinases.

International journal of cancer

Ou DP,Tao YM,Tang FQ,Yang LY

MA5-13587 was used in western blot to examine the effect of hepatitis B virus X protein on hepatocellular carcinoma metastasis

Thu Mar 15 00:00:00 EDT 2007

Matrix metalloproteinase induction in fibrosis and fibrotic nodule formation due to silica inhalation.

American journal of physiology. Lung cellular and molecular physiology

Scabilloni JF,Wang L,Antonini JM,Roberts JR,Castranova V,Mercer RR

MA5-13587 was used in immunohistochemistry to study fibrosis and fibrotic nodule formation following silica inhalation and the role of MMPs

Fri Apr 01 00:00:00 EST 2005

Cardiac morphogenesis: matrix metalloproteinase coordination of cellular mechanisms underlying heart tube formation and directionality of looping.

Developmental dynamics : an official publication of the American Association of Anatomists

Linask KK,Han M,Cai DH,Brauer PR,Maisastry SM

MA5-13587 was used in blocking or activating experiment to study the role of MMPs in cardiac morphogenesis

Fri Jul 01 00:00:00 EDT 2005

Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease.

Gut

Kirkegaard T,Hansen A,Bruun E,Brynskov J

MA5-13587 was used in immunohistochemistry to investigate the expression and the role of matrix metalloproteinases in intestinal fistulae

Sat May 01 00:00:00 EDT 2004

Expression and localisation of matrix metalloproteinases and their natural inhibitors in fistulae of patients with Crohn's disease.

Gut

Kirkegaard T,Hansen A,Bruun E,Brynskov J

MA5-13587 was used in immunohistochemistry to investigate the expression and the role of matrix metalloproteinases in intestinal fistulae

Sat May 01 00:00:00 EDT 2004

Expression of matrix metalloproteinase (MMP)-2 and MMP-9 in breast cancer with a special reference to activator protein-2, HER2, and prognosis.

Clinical cancer research : an official journal of the American Association for Cancer Research

Pellikainen JM,Ropponen KM,Kataja VV,Kellokoski JK,Eskelinen MJ,Kosma VM

MA5-13587 was used in immunohistochemistry to study the prognostic value of MMP2 and MMP9 in breast cancer and their relationship to activator protein-2 and HER2

Mon Nov 15 00:00:00 EST 2004

Comparative study of MMP-2 (matrix metalloproteinase 2) immune expression in normal uterine cervix, intraepithelial neoplasias, and squamous cells cervical carcinoma.

American journal of obstetrics and gynecology

Gaiotto MA,Focchi J,Ribalta JL,Stávale JN,Baracat EC,Lima GR,Guerreiro da Silva ID

MA5-13587 was used in immunohistochemistry to study the expression of MMP-2 in normal uterine cervix, intraepithelial neoplasias, and squamous cells cervical carcinoma

Sat May 01 00:00:00 EDT 2004

A comparative study of MMP-2 in vulvar neoplasms.

Gynecologic oncology

Bovo AC,da Silva ID,Takita LC,Fochi J,Stávale JN,Marks G,de Lima GR

MA5-13587 was used in immunohistochemistry to study MMP-2 protein expressin in various vulvar neoplasms

Sat May 01 00:00:00 EDT 2004

Phase-dependent expression of matrix metalloproteinases and their inhibitors in demyelinating canine distemper encephalitis.

Acta neuropathologica

Miao Q,Baumgärtner W,Failing K,Alldinger S

MA5-13587 was used in immunohistochemistry to study the phase-dependent expression of MMPs and TIMPs in demyelinating canine distemper encephalitis

Sat Nov 01 00:00:00 EST 2003

Derivation and characterization of a Wilms' tumour cell line, WiT 49.

International journal of cancer

Alami J,Williams BR,Yeger H

MA5-13587 was used in western blot to derive and characterize a Wilms' tumour cell line

Mon Nov 10 00:00:00 EST 2003

Matrix metalloproteinases 9 and 2 are necessary for the migration of Langerhans cells and dermal dendritic cells from human and murine skin.

Journal of immunology (Baltimore, Md. : 1950)

Ratzinger G,Stoitzner P,Ebner S,Lutz MB,Layton GT,Rainer C,Senior RM,Shipley JM,Fritsch P,Schuler G,Romani N

MA5-13587 was used in blocking or activating experiment to study the role of MMP2 and MMP9 in the migration of Langerhans cells and dermal dendritic cells from skin

Wed May 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: 72 kDa gelatinase; 72 kDa type IV collagenase; 72kD gelatinase; 72kD type IV collagenase; 72kDa gelatinase; 72kDa type IV collagenase; CLG 4A; Collagenase; collagenase type IV-A; Gelatinase A; Gelatinase alpha; Mat; matrix metallo protease; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); matrix metalloproteinase 2; Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP; MMP-2; MMPs; neutrophil gelatinase; Progelatinase A; TBE-1; TBE1

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Gene Aliases: CLG4; CLG4A; GelA; MMP-2; MMP-II; MMP2; MONA; TBE-1

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UniProt ID: (Human) P08253, (Rat) P33436, (Mouse) P33434

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Entrez Gene ID: (Human) 4313, (Rat) 81686, (Mouse) 17390

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Function(s)

metalloendopeptidase activity serine-type endopeptidase activity protein binding metallopeptidase activity zinc ion binding fibronectin binding peptidase activity hydrolase activity metal ion binding metalloprotease

Process(es)

angiogenesis response to hypoxia blood vessel maturation intramembranous ossification proteolysis embryo implantation extracellular matrix disassembly collagen catabolic process endodermal cell differentiation cellular protein metabolic process positive regulation of innate immune response ephrin receptor signaling pathway face morphogenesis bone trabecula formation cellular response to amino acid stimulus positive regulation of vascular smooth muscle cell proliferation ovarian follicle development ovulation from ovarian follicle luteinization response to oxidative stress negative regulation of cell adhesion female pregnancy parturition aging response to radiation response to mechanical stimulus peripheral nervous system axon regeneration response to activity cell migration positive regulation of cell migration response to retinoic acid response to cytokine response to nicotine response to drug response to hydrogen peroxide positive regulation of apoptotic process response to estrogen negative regulation of vasoconstriction skeletal system morphogenesis tissue remodeling response to electrical stimulus response to hyperoxia prostate gland epithelium morphogenesis cellular response to cytokine stimulus cellular response to interleukin-1 cellular response to estradiol stimulus cellular response to fluid shear stress

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MMP2 Monoclonal Antibody (CA-4001 (CA719E3C)), Biotin

MMP2 Antibody (MA5-13587) in WB

Product Details

MA5-13587

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Neutralization (Neu)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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