Invitrogen

MMP9 Recombinant Rabbit Monoclonal Antibody (JA80-73)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

7 Published Figures

8 References

View all (62) MMP-9 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

MMP9 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

MMP9 Antibody (MA5-32705) in ICC/IF

Immunofluorescence analysis of Matrix metalloproteinase-9 was performed using 70% confluent log phase THP-1 cells differentiated to Macrophages using PMA treatment (100nM, 48 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MMP9 Recombinant Rabbit Monoclonal Antibody (JA80-73) (Product # MA5-32705) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then la... View More

MMP9 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MMP9 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. THP-1 cells were transfected with Matrix metalloproteinase-9 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-MMP9 Recombinant Rabbit Monoclonal Antibody (JA80-73) (Product # MA5-32705). {KD}

Product Details

MA5-32705

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JA80-73

Immunogen

Synthetic peptide within Human MMP9 aa 71-120

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2809982

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

MMP9 (matrix metallopeptidase 9, GELB, CLG4B) is a matrix metalloproteinase, a family of zinc and calcium-dependent endopeptidases that degrade extracellular matrix proteins. MMP9 is secreted as a 92kDa zymogen and cleavage of pro-MMP9 results in the active enzyme with a molecular weight of 82kDa. MMP9 has a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP9 is produced by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells, and is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP9 is supplied by bone marrow-derived cells and contributes to skin carcinogenesis. Further, MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Studies have shown that elevated MMP9 is associated with progression of idiopathic atrial fibrillation and aortic aneurysm.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.