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MOV10 Antibody (MA5-38166) in WB
Western blot analysis of MOV10 in extracts of various cell lines. Samples were incubated with MOV10 Monoclonal antibody (Product # MA5-38166) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-38166
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1817
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 800-900 of human MOV10 (Q9HCE1)
Conjugate
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2898083
Product Specific Information
Positive Samples: U2OS, HepG2, NIH/3T3, U-87MG, Mouse liver, Rat kidney
Immunogen sequence: AATVTSYLKL LLAPSSKKGK ARLSPRSVGV ISPYRKQVEK IRYCITKLDR ELRGLDDIKD LKVGSVEEFQ GQERSVILIS TVRSSQSFVQ LDLDFNLGFL K
Target Information
5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs (PubMed:24726324). Required for microRNA (miRNA)-mediated gene silencing by the RNA-induced silencing complex (RISC). Required for both miRNA-mediated translational repression and miRNA-mediated cleavage of complementary mRNAs by RISC (PubMed:16289642, PubMed:17507929, PubMed:22791714). In cooperation with FMR1, regulates miRNA-mediated translational repression by AGO2 (PubMed:25464849). Restricts retrotransposition of long interspersed element-1 (LINE-1) in cooperation with TUT4 and TUT7 counteracting the RNA chaperonne activity of L1RE1 (PubMed:30122351, PubMed:23093941). Facilitates LINE-1 uridylation by TUT4 and TUT7 (PubMed:30122351). Required for embryonic viability and for normal central nervous system development and function. Plays two critical roles in early brain development: suppresses retroelements in the nucleus by directly inhibiting cDNA synthesis, while regulates cytoskeletal mRNAs to influence neurite outgrowth in the cytosol. May function as a messenger ribonucleoprotein (mRNP) clearance factor (PubMed:24726324). Exhibits antiviral activity against dengue virus (DENV) (PubMed:27974568). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Armitage homolog; capping protein (actin filament) muscle Z-line, alpha 1; functional spliceosome-associated protein 113; Helicase MOV-10; Moloney leukemia virus 10 protein; Mov10, Moloney leukemia virus 10; Mov10, Moloney leukemia virus 10, homolog; Putative helicase MOV-10
Gene Aliases: C77703; Capza1; fSAP113; Gb110; KIAA1631; Mov-10; MOV10
UniProt ID: (Human) Q9HCE1, (Mouse) P23249
Entrez Gene ID: (Human) 4343, (Rat) 310756, (Mouse) 17454
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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