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MPI Antibody (A305-494A) in IP
Detection of human MPI by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MPI antibody A305-494A (lot A305-494A-1) used for IP at 6 µg per reaction. MPI was also immunoprecipitated by rabbit anti-MPI antibody A305-495A. For blotting immunoprecipitated MPI, A305-494A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A305-494A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 25 to 75 of human Mannose-6-phosphate isomerase.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Chimpanzee
Target Information
Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Mannose-6-phosphate isomerase; Phosphohexomutase; Phosphomannose isomerase; phosphomannose isomerase 1; PMI
Gene Aliases: CDG1B; MPI; PMI; PMI1
UniProt ID: (Human) P34949
Entrez Gene ID: (Human) 4351
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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