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MSH2 Antibody (MA5-50645) in ICC/IF
Immunocytochemistry analysis of MSH2 in Daudi cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with MSH2 recombinant monoclonal antibody (Product # MA5-50645) with a dilution of 1:200 in 2% negative goat serum overnight at 4 . Followed by secondary antibody Goat Anti-Mouse IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary antibody was... View More
Product Details
MA5-50645
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
10G3-R
Immunogen
Synthetic peptide within N-terminal residues of Human MSH2 aa 21-70/934.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3094151
Product Specific Information
Positive control: RAW264.7 cell lysate, PC-12 cell lysate, A431 cell lysate, NIH-3T3 cell lysate, mouse testis tissue lysate, mouse heart tissue lysate, Daudi, human colon cancer tissue, human appendix tissue, human breast cancer tissue, human stomach cancer tissue, rat brain tissue, mouse large intestine tissue.
Predicted band size: 105 kDa
Subcellular Location: Nucleus, Chromosome.
Target Information
Plays a role in neurogenesis and neuronal migration. Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA mismatch repair protein Msh2; hMSH2; mismatch repair protein; Msh2p; mutS homolog 2, colon cancer, nonpolyposis type 1; MutS protein homolog 2
Gene Aliases: AI788990; COCA1; FCC1; HNPCC; HNPCC1; LCFS2; MSH2
UniProt ID: (Human) P43246, (Mouse) P43247, (Rat) P54275
Entrez Gene ID: (Human) 4436, (Mouse) 17685, (Rat) 81709
magnesium ion binding
four-way junction DNA binding
Y-form DNA binding
heteroduplex DNA loop binding
double-strand/single-strand DNA junction binding
DNA binding
double-stranded DNA binding
single-stranded DNA binding
protein binding
ATP binding
protein C-terminus binding
ATPase activity
centromeric DNA binding
enzyme binding
protein kinase binding
mismatched DNA binding
guanine/thymine mispair binding
dinucleotide insertion or deletion binding
single guanine insertion binding
single thymine insertion binding
dinucleotide repeat insertion binding
oxidized purine DNA binding
MutLalpha complex binding
protein homodimerization activity
ADP binding
nucleotide binding
damaged DNA binding
DNA-dependent ATPase activity
single base insertion or deletion binding
DNA metabolism protein
meiotic mismatch repair
in utero embryonic development
oxidative phosphorylation
DNA repair
mismatch repair
postreplication repair
double-strand break repair
meiotic gene conversion
cell cycle arrest
reciprocal meiotic recombination
germ cell development
determination of adult lifespan
male gonad development
response to X-ray
response to UV-B
somatic hypermutation of immunoglobulin genes
somatic recombination of immunoglobulin gene segments
B cell mediated immunity
B cell differentiation
intra-S DNA damage checkpoint
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
negative regulation of neuron apoptotic process
maintenance of DNA repeat elements
negative regulation of reciprocal meiotic recombination
isotype switching
negative regulation of DNA recombination
positive regulation of helicase activity
somatic recombination of immunoglobulin genes involved in immune response
cellular response to DNA damage stimulus
cell cycle
intrinsic apoptotic signaling pathway in response to DNA damage
spermatogenesis
response to organic cyclic compound
response to drug
response to amino acid
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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