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MSH6 Antibody (MA5-44646) in ICC/IF
Immunocytochemistry analysis of MSH6 with A549 cells. Cells were fixed in 4% paraformaldehyde (20 min, room temp), permeabilized with 0.1% Triton X-100 in PBS (5 min, room temp), then blocked with 1% BSA in 10% negative goat serum (1 hr, room temp), incubated with recombinant monoclonal MSH6 (Product # MA5-44646) at a dilution of 1:100 (in 1% BSA in PBST overnight at 4°C), followed by Goat Anti-Rabbit IgG H&L 488 at 1:1,000 dilution (PBS used in control). Nuclear DNA was labelled in blue with DAPI, Beta tubulin (red) at 1/100 dilution (ove... View More
Product Details
MA5-44646
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PD00-26
Immunogen
Synthetic peptide within Human MSH6 aa 350-450 (internal sequence).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931104
Target Information
The DNA mismatch repair MSH2/MSH6 heterodimer is responsible for the detection of DNA damage, and may promote apoptosis in normal cells, thus protecting mammals from ultraviolet-induced malignant transformation and hence the prevention of skin cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA mismatch repair protein Msh6; G/T mismatch-binding protein; GTBP; hMSH6; mutS (E. coli) homolog 6; MutS alpha 160kDa subunit; MutS protein homolog 6; MutS-alpha 160 kDa subunit; MutS-alpha 160kDa subunit; p160; Sperm associated protein; sperm-associated protein
Gene Aliases: AU044881; AW550279; GTBP; GTMBP; HNPCC5; HSAP; MSH6; p160
UniProt ID: (Human) P52701, (Mouse) P54276
Entrez Gene ID: (Human) 2956, (Mouse) 17688
magnesium ion binding
four-way junction DNA binding
chromatin binding
double-stranded DNA binding
protein binding
ATP binding
ATPase activity
mismatched DNA binding
guanine/thymine mispair binding
single guanine insertion binding
single thymine insertion binding
oxidized purine DNA binding
MutLalpha complex binding
methylated histone binding
protein homodimerization activity
ADP binding
nucleotide binding
DNA binding
damaged DNA binding
DNA-dependent ATPase activity
single base insertion or deletion binding
DNA metabolism protein
meiotic mismatch repair
DNA repair
mismatch repair
reciprocal meiotic recombination
determination of adult lifespan
intrinsic apoptotic signaling pathway in response to DNA damage
response to UV
viral process
somatic hypermutation of immunoglobulin genes
somatic recombination of immunoglobulin gene segments
maintenance of DNA repeat elements
isotype switching
positive regulation of isotype switching
negative regulation of DNA recombination
positive regulation of helicase activity
intrinsic apoptotic signaling pathway
cellular response to DNA damage stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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