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MTA3 Antibody (PA5-100022) in ICC/IF
Immunofluorescent analysis of MTA3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and MTA3 polyclonal antibody (Product # PA5-100022) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100022
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human MTA3(Accession Q9BTC8), corresponding to amino acid residues N361-K411.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2815552
Product Specific Information
Antibody detects endogenous levels of total MTA3.
Target Information
Plays a role in maintenance of the normal epithelial architecture through the repression of SNAI1 transcription in a histone deacetylase-dependent manner, and thus the regulation of E-cadherin levels. Contributes to transcriptional repression by BCL6. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: metastasis associated 1 family, member 3; metastasis associated 3; metastasis associated family, member 3; metastasis associated gene family, member 3; Metastasis-associated protein MTA3
Gene Aliases: 1110002J22Rik; KIAA1266; mKIAA1266; MTA3
UniProt ID: (Human) Q9BTC8, (Mouse) Q924K8
Entrez Gene ID: (Human) 57504, (Mouse) 116871, (Rat) 100362346
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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