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MTHFD1 Polyclonal Antibody

MTHFD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MTHFD1 Antibody (10794-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using (Product # 10794-1-AP) (MTHFD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MTHFD1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10794-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MTHFD1 Fusion Protein Ag1225 (536-935 aa encoded by BC009806)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: TNDRFLRKI TIGQAPTEKG HTRTAQFDIS VASEIMAVLA LTTSLEDMRE RLGKMVVASS KKGEPVSAED LGVSGALTVL MKDAIKPNLM QTLEGTPVFV HAGPFANIAH GNSSIIADQI ALKLVGPEGF VVTEAGFGAD IGMEKFFNIK CRYSGLCPHV VVLVATVRAL KMHGGGPTVT AGLPLPKAYI QENLELVEKG FSNLKKQIEN ARMFGIPVVV AVNAFKTDTE SELDLISRLS REHGAFDAVK CTHWAEGGKG ALALAQAVQR AAQAPSSFQL LYDLKLPVED KIRIIAQKIY GADDIELLPE AQHKAEVYTK QGFGNLPICM AKTHLSLSHN PEQKGVPTGF ILPIRDIRAS VGAGFLYPLV GTMSTMPGLP TRPCFYDIDL DPETEQVNGL F (536-935 aa encoded by BC009806)

Target Information

This gene encodes a protein that possesses three distinct enzymatic activities, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methenyltetrahydrofolate cyclohydrolase and 10-formyltetrahydrofolate synthetase. Each of these activities catalyzes one of three sequential reactions in the interconversion of 1-carbon derivatives of tetrahydrofolate, which are substrates for methionine, thymidylate, and de novo purine syntheses. The trifunctional enzymatic activities are conferred by two major domains, an aminoterminal portion containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Newcastle Disease Virus Manipulates Mitochondrial MTHFD2-Mediated Nucleotide Metabolism for Virus Replication.

Journal of virology

Tang N,Chen P,Zhao C,Liu P,Tan L,Song C,Qiu X,Liao Y,Liu X,Luo T,Sun Y,Ding C

Thu Mar 30 00:00:00 EDT 2023

Cysteine is a limiting factor for glioma proliferation and survival.

Molecular oncology

Ruiz-Rodado V,Dowdy T,Lita A,Kramp T,Zhang M,Jung J,Dios-Esponera A,Zhang L,Herold-Mende CC,Camphausen K,Gilbert MR,Larion M

Sun May 01 00:00:00 EDT 2022

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Scientific reports

Mitachi K,Ariake K,Shima H,Sato S,Miura T,Maeda S,Ishida M,Mizuma M,Ohtsuka H,Kamei T,Igarashi K,Unno M

Mon Mar 22 00:00:00 EDT 2021

Orthogonal genome-wide screens of bat cells identify MTHFD1 as a target of broad antiviral therapy.

Proceedings of the National Academy of Sciences of the United States of America

Anderson DE,Cui J,Ye Q,Huang B,Tan Y,Jiang C,Zu W,Gong J,Liu W,Kim SY,Yan BG,Sigmundsson K,Lim XF,Ye F,Niu P,Irving AT,Zhang H,Tang Y,Zhou X,Wang Y,Tan W,Wang LF,Tan X

Tue Sep 28 00:00:00 EDT 2021

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Journal of pineal research

Cui L,Zhao X,Jin Z,Wang H,Yang SF,Hu S

Wed Dec 01 00:00:00 EST 2021

Illuminating NAD⁺ Metabolism in Live Cells and In Vivo Using a Genetically Encoded Fluorescent Sensor.

Developmental cell

Zou Y,Wang A,Huang L,Zhu X,Hu Q,Zhang Y,Chen X,Li F,Wang Q,Wang H,Liu R,Zuo F,Li T,Yao J,Qian Y,Shi M,Yue X,Chen W,Zhang Z,Wang C,Zhou Y,Zhu L,Ju Z,Loscalzo J,Yang Y,Zhao Y

Mon Apr 20 00:00:00 EDT 2020

Cytosolic ME1 integrated with mitochondrial IDH2 supports tumor growth and metastasis.

Redox biology

Shao C,Lu W,Du Y,Yan W,Bao Q,Tian Y,Wang G,Ye H,Hao H

Tue Sep 01 00:00:00 EDT 2020

The Human Transient Receptor Potential Melastatin 2 Ion Channel Modulates ROS Through Nrf2.

Scientific reports

Bao L,Festa F,Freet CS,Lee JP,Hirschler-Laszkiewicz IM,Chen SJ,Keefer KA,Wang HG,Patterson AD,Cheung JY,Miller BA

Tue Oct 01 00:00:00 EDT 2019

Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.

Cancer & metabolism

Koufaris C,Nilsson R

Mon Mar 21 00:00:00 EDT 2022

Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.

Cancer & metabolism

Koufaris C,Nilsson R

Mon Mar 21 00:00:00 EDT 2022

Oxidative stress inhibits distant metastasis by human melanoma cells.

Nature

Piskounova E,Agathocleous M,Murphy MM,Hu Z,Huddlestun SE,Zhao Z,Leitch AM,Johnson TM,DeBerardinis RJ,Morrison SJ

Thu Nov 12 00:00:00 EST 2015

Oxidative stress inhibits distant metastasis by human melanoma cells.

Nature

Piskounova E,Agathocleous M,Murphy MM,Hu Z,Huddlestun SE,Zhao Z,Leitch AM,Johnson TM,DeBerardinis RJ,Morrison SJ

Thu Nov 12 00:00:00 EST 2015

Bioinformatics

Protein Aliases: 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase; C-1-tetrahydrofolate synthase, cytoplasmic; C1-tetrahydrofolate synthase; C1-THF synthase; cytoplasmic C-1-tetrahydrofolate synthase; Epididymis secretory sperm binding protein; methenyltetrahydrofolate; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase

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Gene Aliases: Dcs; E430024A07Rik; MTHFC; MTHFD; MTHFD1

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UniProt ID: (Human) P11586, (Mouse) Q922D8

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Entrez Gene ID: (Human) 4522, (Mouse) 108156

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Function(s)

formate-tetrahydrofolate ligase activity methenyltetrahydrofolate cyclohydrolase activity methylenetetrahydrofolate dehydrogenase [NAD(P)+] activity methylenetetrahydrofolate dehydrogenase (NAD+) activity methylenetetrahydrofolate dehydrogenase (NADP+) activity protein binding ATP binding nucleotide binding catalytic activity oxidoreductase activity hydrolase activity ligase activity

Process(es)

histidine biosynthetic process neutrophil homeostasis neural tube closure purine nucleotide biosynthetic process methionine metabolic process one-carbon metabolic process heart development serine family amino acid metabolic process serine family amino acid biosynthetic process methionine biosynthetic process 10-formyltetrahydrofolate biosynthetic process transsulfuration tetrahydrofolate interconversion folic acid metabolic process embryonic neurocranium morphogenesis embryonic viscerocranium morphogenesis oxidation-reduction process somite development metabolic process cellular amino acid biosynthetic process folic acid-containing compound biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MTHFD1 Polyclonal Antibody

MTHFD1 Antibody (10794-1-AP) in IHC (P)

Product Details

10794-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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