Proteintech

MTHFD1L Polyclonal Antibody

MTHFD1L Antibody in Immunocytochemistry (ICC/IF)

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MTHFD1L Antibody (16113-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using (Product # 16113-1-AP) (MTHFD1L antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

MTHFD1L Antibody in Immunohistochemistry (Paraffin) (IHC (P))

MTHFD1L Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

16113-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:100

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MTHFD1L Fusion Protein Ag9036 (427-775 aa encoded by BC017477)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: GRMVVASDK SGQPVTADDL GVTGALTVLM KDAIKPNLMQ TLEGTPVFVH AGPFANIAHG NSSVLADKIA LKLVGEEGFV VTEAGFGADI GMEKFFNIKC RASGLVPNVV VLVATVRALK MHGGGPSVTA GVPLKKEYTE ENIQLVADGC CNLQKQIQIT QLFGVPVVVA LNVFKTDTRA EIDLVCELAK RAGAFDAVPC YHWSVGGKGS VDLARAVREA ASKRSRFQFL YDVQVPIVDK IRTIAQAVYG AKDIELSPEA QAKIDRYTQQ GFGNLPICMA KTHLSLSHQP DKKGVPRDFI LPISDVRASI GAGFIYPLVG TMSTMPGLPT RPCFYDIDLD TETEQVKGLF (427-775 aa encoded by BC017477)

Target Information

One-carbon substituted forms of tetrahydrofolate (THF) are involved in the de novo synthesis of purines and thymidylate and support cellular methylation reactions through the regeneration of methionine from homocysteine. MTHFD1L is an enzyme involved in THF synthesis in mitochondria (Christensen et al., 2005 [PubMed 15611115]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Formate overflow drives toxic folate trapping in MTHFD1 inhibited cancer cells.

Nature metabolism

Green AC,Marttila P,Kiweler N,Chalkiadaki C,Wiita E,Cookson V,Lesur A,Eiden K,Bernardin F,Vallin KSA,Borhade S,Long M,Ghahe EK,Jiménez-Alonso JJ,Jemth AS,Loseva O,Mortusewicz O,Meyers M,Viry E,Johansson AI,Hodek O,Homan E,Bonagas N,Ramos L,Sandberg L,Frödin M,Moussay E,Slipicevic A,Letellier E,Paggetti J,Sørensen CS,Helleday T,Henriksson M,Meiser J

Sat Apr 01 00:00:00 EDT 2023

The SMYD3-MTHFD1L-formate metabolic regulatory axis mediates mitophagy to inhibit M1 polarization in macrophages.

International immunopharmacology

Zhu W,Wang C,Xue L,Liu L,Yang X,Liu Z,Zhang S,Luo D

Thu Dec 01 00:00:00 EST 2022

Extracellular serine empowers epidermal proliferation and psoriasis-like symptoms.

Science advances

Cappello A,Mancini M,Madonna S,Rinaldo S,Paone A,Scarponi C,Belardo A,Zolla L,Zuccotti A,Panatta E,Pallotta S,Annicchiarico-Petruzzelli M,Albanesi C,Cutruzzolà F,Wang L,Jia W,Melino G,Candi E

Fri Dec 16 00:00:00 EST 2022

Purine nucleotide depletion prompts cell migration by stimulating the serine synthesis pathway.

Nature communications

Soflaee MH,Kesavan R,Sahu U,Tasdogan A,Villa E,Djabari Z,Cai F,Tran DH,Vu HS,Ali ES,Rion H,O'Hara BP,Kelekar S,Hallett JH,Martin M,Mathews TP,Gao P,Asara JM,Manning BD,Ben-Sahra I,Hoxhaj G

Mon May 16 00:00:00 EDT 2022

Mitochondria preserve an autarkic one-carbon cycle to confer growth-independent cancer cell migration and metastasis.

Nature communications

Kiweler N,Delbrouck C,Pozdeev VI,Neises L,Soriano-Baguet L,Eiden K,Xian F,Benzarti M,Haase L,Koncina E,Schmoetten M,Jaeger C,Noman MZ,Vazquez A,Janji B,Dittmar G,Brenner D,Letellier E,Meiser J

Mon May 16 00:00:00 EDT 2022

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Journal of pineal research

Cui L,Zhao X,Jin Z,Wang H,Yang SF,Hu S

Wed Dec 01 00:00:00 EST 2021

Melatonin modulates metabolic remodeling in HNSCC by suppressing MTHFD1L-formate axis.

Journal of pineal research

Cui L,Zhao X,Jin Z,Wang H,Yang SF,Hu S

Wed Dec 01 00:00:00 EST 2021

Tumor Reliance on Cytosolic versus Mitochondrial One-Carbon Flux Depends on Folate Availability.

Cell metabolism

Lee WD,Pirona AC,Sarvin B,Stern A,Nevo-Dinur K,Besser E,Sarvin N,Lagziel S,Mukha D,Raz S,Aizenshtein E,Shlomi T

Tue Jan 05 00:00:00 EST 2021

Loss of CHCHD2 and CHCHD10 activates OMA1 peptidase to disrupt mitochondrial cristae phenocopying patient mutations.

Human molecular genetics

Liu YT,Huang X,Nguyen D,Shammas MK,Wu BP,Dombi E,Springer DA,Poulton J,Sekine S,Narendra DP

Wed Jun 03 00:00:00 EDT 2020

MTHFD1L, A Folate Cycle Enzyme, Is Involved in Progression of Colorectal Cancer.

Translational oncology

Agarwal S,Behring M,Hale K,Al Diffalha S,Wang K,Manne U,Varambally S

Fri Nov 01 00:00:00 EDT 2019

Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.

Cell metabolism

Forsström S,Jackson CB,Carroll CJ,Kuronen M,Pirinen E,Pradhan S,Marmyleva A,Auranen M,Kleine IM,Khan NA,Roivainen A,Marjamäki P,Liljenbäck H,Wang L,Battersby BJ,Richter U,Velagapudi V,Nikkanen J,Euro L,Suomalainen A

Tue Dec 03 00:00:00 EST 2019

Increased formate overflow is a hallmark of oxidative cancer.

Nature communications

Meiser J,Schuster A,Pietzke M,Vande Voorde J,Athineos D,Oizel K,Burgos-Barragan G,Wit N,Dhayade S,Morton JP,Dornier E,Sumpton D,Mackay GM,Blyth K,Patel KJ,Niclou SP,Vazquez A

Tue Apr 10 00:00:00 EDT 2018

Increased formate overflow is a hallmark of oxidative cancer.

Nature communications

Meiser J,Schuster A,Pietzke M,Vande Voorde J,Athineos D,Oizel K,Burgos-Barragan G,Wit N,Dhayade S,Morton JP,Dornier E,Sumpton D,Mackay GM,Blyth K,Patel KJ,Niclou SP,Vazquez A

Tue Apr 10 00:00:00 EDT 2018

Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.

Cancer & metabolism

Koufaris C,Nilsson R

Mon Mar 21 00:00:00 EDT 2022

Protein interaction and functional data indicate MTHFD2 involvement in RNA processing and translation.

Cancer & metabolism

Koufaris C,Nilsson R

Mon Mar 21 00:00:00 EDT 2022

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

Cell metabolism

Nikkanen J,Forsström S,Euro L,Paetau I,Kohnz RA,Wang L,Chilov D,Viinamäki J,Roivainen A,Marjamäki P,Liljenbäck H,Ahola S,Buzkova J,Terzioglu M,Khan NA,Pirnes-Karhu S,Paetau A,Lönnqvist T,Sajantila A,Isohanni P,Tyynismaa H,Nomura DK,Battersby BJ,Velagapudi V,Carroll CJ,Suomalainen A

Tue Apr 12 00:00:00 EDT 2016

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

Cell metabolism

Tue Apr 12 00:00:00 EDT 2016

Mitochondrial DNA Replication Defects Disturb Cellular dNTP Pools and Remodel One-Carbon Metabolism.

Cell metabolism

Tue Apr 12 00:00:00 EDT 2016

Targeting MTHFD2 in acute myeloid leukemia.

The Journal of experimental medicine

Pikman Y,Puissant A,Alexe G,Furman A,Chen LM,Frumm SM,Ross L,Fenouille N,Bassil CF,Lewis CA,Ramos A,Gould J,Stone RM,DeAngelo DJ,Galinsky I,Clish CB,Kung AL,Hemann MT,Vander Heiden MG,Banerji V,Stegmaier K

Mon Jun 27 00:00:00 EDT 2016

Serine one-carbon catabolism with formate overflow.

Science advances

Meiser J,Tumanov S,Maddocks O,Labuschagne CF,Athineos D,Van Den Broek N,Mackay GM,Gottlieb E,Blyth K,Vousden K,Kamphorst JJ,Vazquez A

Sat Oct 01 00:00:00 EDT 2016

Serine one-carbon catabolism with formate overflow.

Science advances

Meiser J,Tumanov S,Maddocks O,Labuschagne CF,Athineos D,Van Den Broek N,Mackay GM,Gottlieb E,Blyth K,Vousden K,Kamphorst JJ,Vazquez A

Sat Oct 01 00:00:00 EDT 2016

Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.

Proteomics

Thomas A,Stevens AP,Klein MS,Hellerbrand C,Dettmer K,Gronwald W,Oefner PJ,Reinders J

Tue May 01 00:00:00 EDT 2012

Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.

Proteomics

Thomas A,Stevens AP,Klein MS,Hellerbrand C,Dettmer K,Gronwald W,Oefner PJ,Reinders J

Tue May 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 10-formyl-THF synthetase; Formyltetrahydrofolate synthetase; formyltetrahydrofolate synthetase domain containing 1; Monofunctional C1-tetrahydrofolate synthase, mitochondrial

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Gene Aliases: 2410004L15Rik; AI647056; dJ292B18.2; FTHFSDC1; MTC1THFS; MTHFD1L

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UniProt ID: (Human) Q6UB35, (Mouse) Q3V3R1

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Entrez Gene ID: (Human) 25902, (Rat) 361472, (Mouse) 270685

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Function(s)

formate-tetrahydrofolate ligase activity methenyltetrahydrofolate cyclohydrolase activity methylenetetrahydrofolate dehydrogenase (NADP+) activity ATP binding protein homodimerization activity molecular_function nucleotide binding catalytic activity ligase activity

Process(es)

one-carbon metabolic process folic acid-containing compound metabolic process biological_process folic acid-containing compound biosynthetic process formate metabolic process tetrahydrofolate metabolic process oxidation-reduction process 10-formyltetrahydrofolate biosynthetic process neural tube closure tetrahydrofolate interconversion folic acid metabolic process embryonic neurocranium morphogenesis embryonic viscerocranium morphogenesis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MTHFD1L Polyclonal Antibody

MTHFD1L Antibody (16113-1-AP) in ICC/IF

Product Details

16113-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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