Invitrogen

MUC1 Monoclonal Antibody (E29)

MUC1 Antibody in Immunocytochemistry (ICC/IF)

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MUC1 Antibody (MA5-14077) in ICC/IF

Immunofluorescence analysis of MUC1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with MUC1 Monoclonal Antibody (LH39) (Product # MA5-14077) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

MUC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-14077

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:400-1:800

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

E29

Immunogen

Delipidated extract of human milk fat globule membranes

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10985258

Product Specific Information

MA5-14077 targets Epithelial Membrane Antigen in IHC (P) applications and shows reactivity with Human samples.

The MA5-14077 immunogen is delipidated extract of human milk fat globule membranes.

Target Information

MUC1 (Mucin 1, Episialin, MAM-6, CA 15-3, PEM and EMA) is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. MUC1 is a transmembrane glycoprotein with a large mucin-like extracellular domain that matures through several intermediate forms generated by proteolysis, and sequential addition and processing of numerous O-linked glycans that are heavily sialylated. MUC1 is highly polymorphic and each allele encodes a product that contains a different number of repeats (between 30 and 90) leading to large differences in molecular weight of the protein. MUC1 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells, and is expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. Overexpression, aberrant intracellular localization, and changes in glycosylation of the MUC1 protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of the MUC1 gene have been reported, but the full-length nature of only some has been determined. Transgenic MUC-1 has been shown to associate with all four cebB receptors and localize with erbB1 (EGFR) in lactating glands.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Integrin αvβ6 as a Target for Tumor-Specific Imaging of Vulvar Squamous Cell Carcinoma and Adjacent Premalignant Lesions.

Cancers

Huisman BW,Cankat M,Bosse T,Vahrmeijer AL,Rissmann R,Burggraaf J,Sier CFM,van Poelgeest MIE

MA5-14077 was used in Immunohistochemistry to conclude that integrin αvβ6 allows for the most robust discrimination of vulvar squamous cell carcinomas and adjacent premalignant lesions compared to surrounding healthy tissue.

Mon Nov 29 00:00:00 EST 2021

Oncocytic papillary renal cell carcinoma: A clinicopathological and genetic analysis and indolent clinical course in 14 cases.

Pathology, research and practice

Han G,Yu W,Chu J,Liu Y,Jiang Y,Li Y,Zhang W

MA514077 was used in immunohistochemistry - paraffin section to characterize cases of oncocytic papillary renal cell carcinoma

Sun Jan 01 00:00:00 EST 2017

Primary acinic cell carcinoma of the breast: a case report and review of the literature.

International journal of surgical pathology

Zhao Y,Li W,Lang R,Yang Y,Gao X,Zheng Y,Zhang C,Fu X,Fu L

MA5-14077 was used in immunohistochemistry to report the case of primary acinic cell carcinoma of the breast in a 38-year-old woman.

Tue Apr 01 00:00:00 EDT 2014

Pancreatic mucinous cystic tumor in Turner syndrome: How a tumor bends to a genetic disease.

International journal of surgery case reports

Pizzi M,Pennelli G,Merante-Boschin I,Fassan M,Pelizzo MR,Rugge M

MA5-14077 was used in immunohistochemistry to report on a case of pancreatic mucinous cystic tumor in a patient with Turner syndrome

Fri Nov 08 00:00:00 EST 2013

Diagnostic and therapeutic strategy and the most efficient prognostic factors of breast malignant fibrous histiocytoma.

Scientific reports

Qiu SQ,Wei XL,Huang WH,Wu MY,Qin YS,Li YK,Zhang GJ

MA5-14077 was used in immunohistochemistry - paraffin section to report the clinicopathological features of 9 breast malignant fibrous histiocytoma patients

Fri Feb 14 00:00:00 EST 2014

An immunohistochemical study of the origin of the solid strand in syringoma, using carcinoembryonic antigen, epithelial membrane antigen, and cytokeratin 5.

International journal of dermatology

Kim BC,Park EJ,Kwon IH,Cho HJ,Park HR,Kim KH,Kim KJ

MA5-14077 was used in immunohistochemistry to study the precise cellular origin of syringoma

Sun Jul 01 00:00:00 EDT 2012

Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.

Diagnostic pathology

Yin WH,Li J,Chan JK

MA5-14077 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression

Thu Apr 12 00:00:00 EDT 2012

Anaplastic large cell lymphoma with paraneoplastic leukocytosis: a clinicopathological analysis of five cases.

APMIS : acta pathologica, microbiologica, et immunologica Scandinavica

Chang IW,Chen HK,Ma MC,Huang WT

MA5-14077 was used in immunohistochemistry to report on five cases of anaplastic large cell lymphoma with paraneoplastic leukocytosis

Tue Nov 01 00:00:00 EDT 2011

Atypical teratoid/rhabdoid tumors may show morphological and immunohistochemical features seen in choroid plexus tumors.

Neuropathology : official journal of the Japanese Society of Neuropathology

Schittenhelm J,Nagel C,Meyermann R,Beschorner R

MA5-14077 was used in immunohistochemistry to study the morphological and immunohistochemical features of atypical teratoid/rhabdoid tumors

Sat Oct 01 00:00:00 EDT 2011

Primary choroid plexus papilloma of the sacral nerve roots.

Journal of neurosurgery. Spine

Boldorini R,Panzarasa G,Girardi P,Monga G

MA5-14077 was used in immunohistochemistry to report on a case of choroid plexus papilloma of the sacral nerve roots

Thu Jan 01 00:00:00 EST 2009

Infratentorial ependymomas: prognostic factors and outcome analysis in a multi-center retrospective series of 106 adult patients.

Acta neurochirurgica

Guyotat J,Metellus P,Giorgi R,Barrie M,Jouvet A,Fevre-Montange M,Chinot O,Durand A,Figarella-Branger D

MA5-14077 was used in immunohistochemistry to evaluate the major prognostic factors for posterior fossa ependymomas

Sat Aug 01 00:00:00 EDT 2009

Dedifferentiated Liposarcoma of the Retroperitoneum with Extensive Leiomyosarcomatous Differentiation and beta-Human Chorionic Gonadotropin Production.

Sarcoma

Russell MJ,Flynt FL,Harroff AL,Fadare O

MA5-14077 was used in immunohistochemistry to report a clinical case of dedifferentiated liposarcoma of the retroperitoneum with unique features

Thu Jul 14 00:00:00 EDT 2011

An immunohistochemical profile of giant cell carcinoma of the larynx.

Auris, nasus, larynx

Gurbuz Y,Kose N,Aydin O,Ozturk M

MA5-14077 was used in immunohistochemistry to report on a case of giant cell carcinoma of the larynx

Sat Sep 01 00:00:00 EDT 2007

Anti-tumor effect of the anti-KL-6/MUC1 monoclonal antibody through exposure of surface molecules by MUC1 capping.

Cancer science

Doi M,Yokoyama A,Kondo K,Ohnishi H,Ishikawa N,Hattori N,Kohno N

MA5-14077 was used in blocking or activating experiment to study the mechanism underlying the anti-tumor effects of an anti-KL-6/MUC1 monoclonal antibody

Mon May 01 00:00:00 EDT 2006

Recurrent solitary fibrous tumour in the cerebellopontine angle.

Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia

Bikmaz K,Cosar M,Kurtkaya-Yapicier O,Iplikcioglu AC,Gokduman CA

MA5-14077 was used in immunohistochemistry to report a clinical case of solitary fibrous tumour in the cerebellopontine angle

Thu Sep 01 00:00:00 EDT 2005

Prognostic significance of mucin expression in gastric carcinoma.

Digestive diseases and sciences

Kocer B,Soran A,Kiyak G,Erdogan S,Eroglu A,Bozkurt B,Solak C,Cengiz O

MA5-14077 was used in immunohistochemistry to investigate the prognostic value of mucin in the gastric carcinoma

Tue Jun 01 00:00:00 EDT 2004

A confirmatory report for the close interaction of Helicobacter pylori with gastric epithelial MUC5AC expression.

Journal of clinical gastroenterology

Kocer B,Ulas M,Ustundag Y,Erdogan S,Karabeyoglu M,Yldrm O,Unal B,Cengiz O,Soran A

MA5-14077 was used in immunohistochemistry to study the effect of Helicobacter pylori on gastric mucin expression

Thu Jul 01 00:00:00 EDT 2004

Collagenous fibroma (desmoplastic fibroblastoma).

Yonsei medical journal

Dagli M,Eryilmaz A,Acar A,Kulacoglu S,Akmansu H

MA5-14077 was used in immunohistochemistry to report on a patient with collagenous fibroma

Sun Oct 31 00:00:00 EDT 2004

Expression of membrane-associated mucins MUC1 and MUC4 in major human salivary glands.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Liu B,Lague JR,Nunes DP,Toselli P,Oppenheim FG,Soares RV,Troxler RF,Offner GD

MA5-14077 was used in Immunohistochemistry to show that membrane-associated mucins are produced in both parotid and submandibular glands and that they are expressed in different cell types than gel-forming mucins.

Sat Jun 01 00:00:00 EDT 2002

Bioinformatics

Protein Aliases: Breast carcinoma-associated antigen DF3; CA 15-3; Cancer antigen 15-3; Carcinoma-associated mucin; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; J19; KL-6; Krebs von den Lungen-6; Medullary cystic kidney disease, autosomal dominant; MUC-1; MUC1-alpha; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin

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Gene Aliases: ADMCKD; ADMCKD1; CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1; MUC1/ZD; PEM; PEMT; PUM

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UniProt ID: (Human) P15941, (Mouse) Q02496

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Entrez Gene ID: (Human) 4582, (Mouse) 17829

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding p53 binding transcription cofactor activity protein binding cell adhesion molecule

Process(es)

DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator negative regulation of transcription by competitive promoter binding O-glycan processing negative regulation of cell adhesion mediated by integrin positive regulation of transcription from RNA polymerase II promoter in response to stress regulation of transcription from RNA polymerase II promoter in response to stress positive regulation of histone H4 acetylation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MUC1 Monoclonal Antibody (E29)

MUC1 Antibody (MA5-14077) in ICC/IF

Product Details

MA5-14077

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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