Invitrogen

MUPP1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

21 Published Figures

10 References

View all (13) MUPP1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

MUPP1 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 24

MUPP1 Antibody (42-2700) in IHC

Immunofluorescent staining of Schimdt-Lanterman incisures in fixed mouse sciatic nerve using Zymed Rb anti-MUPP1 (Product # 42-2700). Image courtesy of Dr. James Nagy, University of Manitoba, Winnipeg, Canada.

MUPP1 Antibody in Immunocytochemistry (ICC/IF)

MUPP1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

MUPP1 Antibody — Generation of global and conditional Mpdz knockout mice A Schematic representation of the Mpdz gene locus and the translated protein. The numbered boxes represent exons 1-47 of the wild-type allele. The relative position of the translational start and stop sites is indicated at exon 2 and exon 47, respectively. A gene-trap cassette (beta-geo) was inserted into intron 11-12 leading to a stop signal that truncates the Mpdz protein after the third PDZ domain. B Western blotting to detect Mpdz protein in brain lysates of littermate wild-type and global knockout Mpdz mice. C Schematic drawing of the conditional Mpdz gene targeting strategy. LoxP sites flanking exon 4 and a neomycin resistance cassette were inserted by homologous recombination. Transgenic mice were crossed with Flp deleter mice to remove the FRT-flanked neomycin cassette. Cre recombinase removes exons 4 and 5 leading to a frameshift and a nonsense mutation. This truncates the Mpdz protein after the L27 domain. D Western blotting to detect Mpdz protein in brain lysates of Mpdz fl/fl and CMV-Cre; Mpdz Delta/Delta mice. E, F Kaplan-Meier survival analysis of Mpdz -/- vs. Mpdz +/+ mice ( n > 41 mice per genotype) and CMV-Cre; Mpdz Delta/Delta vs. Mpdz fl/fl mice ( n > 48 mice per genotype). G, H Representative images of Mpdz -/- and Mpdz +/+ mice at postnatal day 27 (P27) and CMV-Cre; Mpdz Delta/Delta and Mpdz fl/fl mice at P24. Arrows indicate the enlarged and dome-shaped skull and the enlarged brain hemispheres. Sour {KO}

Product Details

42-2700

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Gerbil, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminal region of mouse MUPP1

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533517

Target Information

MUPP1 (multi PDZ domain protein 1, MPDZ) was discovered as an interacting partner with the 5-HT2C receptor1, and was found to co-localize with 5-HT2A or 5-HT2C receptors in all regions of the mouse brain, including the choroid plexus. MUPP1 contains 13 PDZ domains but no apparent catalytic domain. MUPP1 is concentrated in tight junctions of polarized epithelial cells and binds to claudin-1 and junctional adhesion molecule (JAM). MUPP1 may therefore function as a multivalent scaffold protein that recruits various proteins to tight junctions. MUPP1 also interacts with claudin-8 and may play a role in tight junction barrier function.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ADAMTS1, MPDZ, MVD, and SEZ6: candidate genes for autosomal recessive nonsyndromic hearing impairment.

European journal of human genetics : EJHG

Bharadwaj T,Schrauwen I,Rehman S,Liaqat K,Acharya A,Giese APJ,Nouel-Saied LM,Nasir A,Everard JL,Pollock LM,Zhu S,Bamshad MJ,Nickerson DA,Ali RH,Ullah A,Wali A,Ali G,Santos-Cortez RLP,Ahmed ZM,McDermott BM,Ansar M,Riazuddin S,Ahmad W,Leal SM

Published figure using MUPP1 polyclonal antibody (Product # 42-2700) in Immunohistochemistry

Sat Jan 01 00:00:00 EST 2022

MPDZ promotes DLL4-induced Notch signaling during angiogenesis.

eLife

Tetzlaff F,Adam MG,Feldner A,Moll I,Menuchin A,Rodriguez-Vita J,Sprinzak D,Fischer A

42-2700 was used in Western Blotting to identify multiple PDZ domain protein as a novel modulator of Notch signaling by controlling ligand recruitment to adherens junctions.

Thu Apr 05 00:00:00 EDT 2018

Loss of Mpdz impairs ependymal cell integrity leading to perinatal-onset hydrocephalus in mice.

EMBO molecular medicine

Feldner A,Adam MG,Tetzlaff F,Moll I,Komljenovic D,Sahm F,Bäuerle T,Ishikawa H,Schroten H,Korff T,Hofmann I,Wolburg H,von Deimling A,Fischer A

Published figure using MUPP1 polyclonal antibody (Product # 42-2700) in Western Blot

Sat Jul 01 00:00:00 EDT 2017

Loss of Mpdz impairs ependymal cell integrity leading to perinatal-onset hydrocephalus in mice.

EMBO molecular medicine

Feldner A,Adam MG,Tetzlaff F,Moll I,Komljenovic D,Sahm F,Bäuerle T,Ishikawa H,Schroten H,Korff T,Hofmann I,Wolburg H,von Deimling A,Fischer A

Published figure using MUPP1 polyclonal antibody (Product # 42-2700) in Western Blot

Sat Jul 01 00:00:00 EDT 2017

The effector and scaffolding proteins AF6 and MUPP1 interact with connexin36 and localize at gap junctions that form electrical synapses in rodent brain.

The European journal of neuroscience

Li X,Lynn BD,Nagy JI