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MUSK Antibody (PA1-1741) in ICC/IF
Immunofluorescent analysis of muscle-specific kinase (MuSK) (green) in C2C12 mouse myoblast cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained without (left panel) or with a MuSK polyclonal antibody (Product # PA1-1741, right panel), at a dilution of 1:20 in blocking buffer for at least 1 hour at room temperature, and then incubated with a DyLight 488 goat anti-rabbit IgG secondary... View More
Product Details
PA1-1741
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein containing residues 210-304 from the extracellular domain of rat MuSK
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2147253
Product Specific Information
PA1-1741 detects a predominant band at ~98kD. Other lower MW nonspecific bands of unknown identity were also detected and are likely to be proteolytic fragments.
Target Information
MUSK plays an important and selective role in many aspects of skeletal muscle development and function through the initial step of postsynaptic differentiation and formation of end-plate bands in cooperation with Rapsyn. Communication between cells is often mediated by receptors on the surface of the target cell that are triggered by specific ligands released by other cells. Receptor tyrosine kinase activity is strictly regulated by the binding of the appropriate ligand to the extracellular active site of the receptor. MuSK has been shown to be part of the agrin signaling complex and plays an important role in the development and architectural maintenance of the neuromuscular junction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: MGC126323; MGC126324; muscle localized kinase 2; muscle specific kinase (neural fold/somite kinase 1); Muscle, skeletal receptor tyrosine protein kinase; Muscle, skeletal receptor tyrosine-protein kinase; muscle, skeletal, receptor tyrosine kinase; muscle-specific kinase receptor; muscle-specific protein kinase secretory isoform; muscle-specific tyrosine kinase receptor MuSK; Muscle-specific tyrosine protein kinase receptor; Muscle-specific tyrosine-protein kinase receptor; MuSK; RP23-170A3.1
Gene Aliases: CMS9; FADS; Mdk4; Mlk; MUSK; Nsk1; Nsk2; Nsk3
UniProt ID: (Human) O15146, (Mouse) Q61006, (Rat) Q62838
Entrez Gene ID: (Human) 4593, (Mouse) 18198, (Rat) 81725
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
protein binding
ATP binding
metal ion binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein kinase binding
PDZ domain binding
transmembrane signal receptor
positive regulation of protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
multicellular organism development
neuromuscular junction development
memory
regulation of synaptic growth at neuromuscular junction
positive regulation of gene expression
peptidyl-tyrosine phosphorylation
cell differentiation
protein autophosphorylation
skeletal muscle acetylcholine-gated channel clustering
positive regulation of protein geranylgeranylation
regulation of transcription, DNA-templated
protein phosphorylation
signal transduction
negative regulation of gene expression
phosphorylation
positive regulation of synaptic transmission, cholinergic
receptor clustering
positive regulation of neuron apoptotic process
positive regulation of synaptic growth at neuromuscular junction
positive regulation of peptidyl-tyrosine phosphorylation
response to electrical stimulus
long-term synaptic potentiation
response to muscle activity
response to axon injury
retina development in camera-type eye
cochlea development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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