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Product Details
19650-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Not Applicable
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
MUTYH Fusion Protein Ag7526 (187-535 aa encoded by BC003178)
Conjugate
Form
Liquid
Concentration
0.28 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: MPRTAETLQ QLLPGVGRYT AGAIASIAFG QATGVVDGNV ARVLCRVRAI GADPSSTLVS QQLWGLAQQL VDPARPGDFN QAAMELGATV CTPQRPLCSQ CPVESLCRAR QRVEQEQLLA SGSLSGSPDV EECAPNTGQC HLCLPPSEPW DQTLGVVNFP RKASRKPPRE ESSATCVLEQ PGALGAQILL VQRPNSGLLA GLWEFPSVTW EPSEQLQRKA LLQELQRWAG PLPATHLRHL GEVVHTFSHI KLTYQVYGLA LEGQTPVTTV PPGARWLTQE EFHTAAVSTA MKKVFRVYQG QQPGTCMGSK RSQVSSPCSR KKPRMGQQVL DNFFRSHIST DAHSLNSAAQ (187-535 aa encoded by BC003178)
Target Information
MYH (mutY homolog (E. coli )) is a DNA glycosylase mismatch repair enzyme that in conjunction with mutM (OGG1), cleaves adenine residues paired with either oxidized (8-hydroxyguanines) or non-modified guanines in order to correct A/G and A/C mismatches. Repair of most modified and mispaired bases in the genome is initiated by DNA glycosylases, which bind and cleave N-glycosyl bonds to initiate base excision repair. MYH is crucial for the avoidance of mutations resulting from oxidative DNA damage. Multiple N-terminal splice variants of MYH exist in mammalian cells. Increasing levels of MYH in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. Biallelic MYH germ-line mutations predispose humans to colorectal adenomas and carcinomas. MYH is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: A/G-specific adenine DNA glycosylase; Adenine DNA glycosylase; adenine-DNA glycosylase; hMYH; MGC4416; mMYH; MutY homolog; mutY homolog alpha; mutY-like protein; OTTHUMP00000009098; OTTHUMP00000009102; rMYH; RP4-534D1.2; the full-length form type A, 5' region
Gene Aliases: 5730495A01Rik; MUTYH; Mutyha; Mutyhalpha; Mutyhb; Mutyhbeta; Mutyhc; MYH
UniProt ID: (Human) Q9UIF7, (Mouse) Q99P21, (Rat) Q8R5G2
Entrez Gene ID: (Human) 4595, (Mouse) 70603, (Rat) 170841
DNA binding
protein binding
DNA N-glycosylase activity
MutLalpha complex binding
MutLbeta complex binding
MutSalpha complex binding
MutSbeta complex binding
metal ion binding
4 iron, 4 sulfur cluster binding
purine-specific mismatch base pair DNA N-glycosylase activity
catalytic activity
uracil DNA N-glycosylase activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
single-strand selective uracil DNA N-glycosylase activity
DNA-3-methylbase glycosylase activity
iron-sulfur cluster binding
nicotinamide riboside hydrolase activity
nicotinic acid riboside hydrolase activity
deoxyribonucleoside 5'-monophosphate N-glycosidase activity
DNA glycosylase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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