Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
MVP Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
MVP Antibody (PA5-84667) in WB
Knockout of MVP was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR909609_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of MVP was performed by loading 30 µg of A549 wild type (Lane 1), A549 Cas9 (Lane 2) and A549 MVP KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ 3 to 8%, Tris-Acetate, 1.0 mm, Mini Protein Gel (Product # EC6695BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB... View More
Product Details
PA5-84667
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human MVP. Recombinant protein control fragment (Product #RP-105340).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Anitgen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2791818
Product Specific Information
Immunogen sequence: KVSHQAGDHW LIRGPLEYVP SAKVEVVEER QAIPLDENEG IYVQDVKTGK VRAVIGSTYM LTQDEVLWEK ELPPGVEELL NKGQDPLAD
Target Information
This gene encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: Lung resistance-related protein; Major vault protein; MVP; MVP1; Testicular Secretory Protein Li 30; VAULT1; vRNP
Gene Aliases: LRP; MVP; VAULT1
UniProt ID: (Human) Q14764
Entrez Gene ID: (Human) 9961
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support