Proteintech

MYOD1 Polyclonal Antibody

MYOD1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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MYOD1 Antibody (18943-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded mouse embryo tissue slide using 18943-1-AP ( MYOD1 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Product Image Gallery

Product Details

18943-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 51 publications 51 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 14 publications 14 publications

Flow Cytometry (Flow)

Assay-dependent

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MYOD1 Fusion Protein Ag13512 (1-320 aa encoded by BC064493)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MELLSPPLR DVDLTAPDGS LCSFATTDDF YDDPCFDSPD LRFFEDLDPR LMHVGALLKP EEHSHFPAAV HPAPGAREDE HVRAPSGHHQ AGRCLLWACK ACKRKTTNAD RRKAATMRER RRLSKVNEAF ETLKRCTSSN PNQRLPKVEI LRNAIRYIEG LQALLRDQDA APPGAAAAFY APGPLPPGRG GEHYSGDSDA SSPRSNCSDG MMDYSGPPSG ARRRNCYEGA YYNEAPSEPR PGKSAAVSSL DCLSSIVERI STESPAAPAL LLADVPSESP PRRQEAAAPS EGESSGDPTQ SPDAAPQCPA GANPNPIYQV L (1-320 aa encoded by BC064493)

Target Information

The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Tue Oct 15 00:00:00 EDT 2019

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YAP1-Mediated Suppression of USP31 Enhances NFκB Activity to Promote Sarcomagenesis.

Cancer research

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Tue May 15 00:00:00 EDT 2018

YAP1-Mediated Suppression of USP31 Enhances NFκB Activity to Promote Sarcomagenesis.

Cancer research

Ye S,Lawlor MA,Rivera-Reyes A,Egolf S,Chor S,Pak K,Ciotti GE,Lee AC,Marino GE,Shah J,Niedzwicki D,Weber K,Park PMC,Alam MZ,Grazioli A,Haldar M,Xu M,Perry JA,Qi J,Eisinger-Mathason TSK

Tue May 15 00:00:00 EDT 2018

YAP1-Mediated Suppression of USP31 Enhances NFκB Activity to Promote Sarcomagenesis.

Cancer research

Ye S,Lawlor MA,Rivera-Reyes A,Egolf S,Chor S,Pak K,Ciotti GE,Lee AC,Marino GE,Shah J,Niedzwicki D,Weber K,Park PMC,Alam MZ,Grazioli A,Haldar M,Xu M,Perry JA,Qi J,Eisinger-Mathason TSK

Tue May 15 00:00:00 EDT 2018

Effects of Dandelion Extract on the Proliferation of Rat Skeletal Muscle Cells and the Inhibition of a Lipopolysaccharide-Induced Inflammatory Reaction.

Chinese medical journal

Liu Q,Zhao H,Gao Y,Meng Y,Zhao XX,Pan SN

Fri Jul 20 00:00:00 EDT 2018

Effects of Dandelion Extract on the Proliferation of Rat Skeletal Muscle Cells and the Inhibition of a Lipopolysaccharide-Induced Inflammatory Reaction.

Chinese medical journal

Liu Q,Zhao H,Gao Y,Meng Y,Zhao XX,Pan SN

Fri Jul 20 00:00:00 EDT 2018

Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.

Journal of agricultural and food chemistry

Zhang L,Gong H,Sun Q,Zhao R,Jia Y

Wed Jan 17 00:00:00 EST 2018

Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.

Journal of agricultural and food chemistry

Zhang L,Gong H,Sun Q,Zhao R,Jia Y

Wed Jan 17 00:00:00 EST 2018

Galectin-3- Mediated Transdifferentiation of Pulmonary Artery Endothelial Cells Contributes to Hypoxic Pulmonary Vascular Remodeling.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Zhang L,Li YM,Zeng XX,Wang XY,Chen SK,Gui LX,Lin MJ

Tue Dec 11 00:00:00 EST 2018

Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP.

Scientific reports

Bi Y,Hua Z,Liu X,Hua W,Ren H,Xiao H,Zhang L,Li L,Wang Z,Laible G,Wang Y,Dong F,Zheng X

Wed Aug 17 00:00:00 EDT 2016

Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP.

Scientific reports

Bi Y,Hua Z,Liu X,Hua W,Ren H,Xiao H,Zhang L,Li L,Wang Z,Laible G,Wang Y,Dong F,Zheng X

Wed Aug 17 00:00:00 EDT 2016

Optogenetic clustering of CNK1 reveals mechanistic insights in RAF and AKT signalling controlling cell fate decisions.

Scientific reports

Fischer A,Warscheid B,Weber W,Radziwill G

Wed Nov 30 00:00:00 EST 2016

Optogenetic clustering of CNK1 reveals mechanistic insights in RAF and AKT signalling controlling cell fate decisions.

Scientific reports

Fischer A,Warscheid B,Weber W,Radziwill G

Wed Nov 30 00:00:00 EST 2016

Regulating myogenic differentiation of mesenchymal stem cells using thermosensitive hydrogels.

Acta biomaterialia

Xu Y,Li Z,Li X,Fan Z,Liu Z,Xie X,Guan J

Thu Oct 01 00:00:00 EDT 2015

Regulating myogenic differentiation of mesenchymal stem cells using thermosensitive hydrogels.

Acta biomaterialia

Xu Y,Li Z,Li X,Fan Z,Liu Z,Xie X,Guan J

Thu Oct 01 00:00:00 EDT 2015

Prostaglandin E2 promotes proliferation of skeletal muscle myoblasts via EP4 receptor activation.

Cell cycle (Georgetown, Tex.)

Mo C,Zhao R,Vallejo J,Igwe O,Bonewald L,Wetmore L,Brotto M

Thu Feb 25 00:00:00 EST 2016

Prostaglandin E2 promotes proliferation of skeletal muscle myoblasts via EP4 receptor activation.

Cell cycle (Georgetown, Tex.)

Mo C,Zhao R,Vallejo J,Igwe O,Bonewald L,Wetmore L,Brotto M

Thu Feb 25 00:00:00 EST 2016

Bioinformatics

Protein Aliases: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; Myoblast determination protein 1; Myogenic factor 3

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Gene Aliases: AI503393; BHLHC1; MYF3; MYOD; Myod-1; MYOD1; PUM

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UniProt ID: (Human) P15172, (Mouse) P10085, (Rat) Q02346

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Entrez Gene ID: (Human) 4654, (Mouse) 17927, (Rat) 337868

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription coactivator activity protein binding transcription factor binding chromatin DNA binding ubiquitin protein ligase binding nuclear hormone receptor binding protein heterodimerization activity E-box binding DNA binding transcription factor activity, sequence-specific DNA binding enzyme binding sequence-specific DNA binding protein dimerization activity basic helix-loop-helix transcription factor

Process(es)

regulation of alternative mRNA splicing, via spliceosome regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein phosphorylation muscle organ development myoblast fate determination skeletal muscle tissue development myoblast fusion cellular response to starvation myotube cell development myotube differentiation involved in skeletal muscle regeneration skeletal muscle cell differentiation muscle cell fate commitment positive regulation of skeletal muscle tissue regeneration regulation of RNA splicing skeletal muscle fiber adaptation histone H3 acetylation histone H4 acetylation positive regulation of myoblast differentiation positive regulation of transcription from RNA polymerase II promoter skeletal muscle fiber development positive regulation of skeletal muscle fiber development positive regulation of muscle cell differentiation cellular response to glucocorticoid stimulus cellular response to estradiol stimulus cellular response to oxygen levels positive regulation of myoblast fusion negative regulation of myoblast proliferation transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development myotube differentiation cell differentiation skeletal muscle tissue regeneration myoblast differentiation positive regulation of transcription, DNA-templated striated muscle cell differentiation cardiac muscle cell differentiation skeletal muscle atrophy response to muscle stretch

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MYOD1 Polyclonal Antibody

MYOD1 Antibody (18943-1-AP) in IHC (P)

Product Details

18943-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

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Not applicable (N/A)

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