Proteintech

Mast Cell Chymase Polyclonal Antibody

Mast Cell Chymase Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Mast Cell Chymase Antibody (18189-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using (Product # 18189-1-AP) (Mast Cell Chymase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Mast Cell Chymase Antibody in Western Blot (WB)

Mast Cell Chymase Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

18189-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Mast Cell Chymase Fusion Protein Ag12909 (1-247 aa encoded by BC069370)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MLLLPLPLL LFLLCSRAEA GEIIGGTECK PHSRPYMAYL EIVTSNGPSK FCGGFLIRRN FVLTAAHCAG RSITVTLGAH NITEEEDTWQ KLEVIKQFRH PKYNTSTLHH DIMLLKLKEK ASLTLAVGTL PFPSQFNFVP PGRMCRVAGW GRTGVLKPGS DTLQEVKLRL MDPQACSHFR DFDHNLQLCV GNPRKTKSAF KGDSGGPLLC AGVAQGIVSY GRSDAKPPAV FTRISHYRPW INQILQAN (1-247 aa encoded by BC069370)

Target Information

This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11. 2 in a cluster of genes encoding other proteases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endothelial Cells Mediated by UCP2 Control the Neurogenic-to-Astrogenic Neural Stem Cells Fate Switch During Brain Development.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang W,Su L,Wang Y,Li C,Ji F,Jiao J

Wed Jun 01 00:00:00 EDT 2022

Endothelial Cells Mediated by UCP2 Control the Neurogenic-to-Astrogenic Neural Stem Cells Fate Switch During Brain Development.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Wang W,Su L,Wang Y,Li C,Ji F,Jiao J

Wed Jun 01 00:00:00 EDT 2022

Characterization of CD41⁺ cells in the lymph node.

Frontiers in immunology

Dai L,Uehara M,Li X,LaBarre BA,Banouni N,Ichimura T,Lee-Sundlov MM,Kasinath V,Sullivan JA,Ni H,Barone F,Giannini S,Bahmani B,Sage PT,Patsopoulos NA,Tsokos GC,Bromberg JS,Hoffmeister K,Jiang L,Abdi R

Tue Aug 30 00:00:00 EDT 2022

Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.

International journal of molecular sciences

Yamanaka-Takaichi M,Mizukami Y,Sugawara K,Sunami K,Teranishi Y,Kira Y,Paus R,Tsuruta D

Tue Mar 09 00:00:00 EST 2021

Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood.

Proceedings of the National Academy of Sciences of the United States of America

Mackey E,Thelen KM,Bali V,Fardisi M,Trowbridge M,Jordan CL,Moeser AJ

Tue Sep 22 00:00:00 EDT 2020

Stabilization of Perivascular Mast Cells by Endothelial CNP (C-Type Natriuretic Peptide).

Arteriosclerosis, thrombosis, and vascular biology

Chen W,Werner F,Illerhaus A,Knopp T,Völker K,Potapenko T,Hofmann U,Frantz S,Baba HA,Rösch M,Zernecke A,Karbach S,Wenzel P,Kuhn M

Sun Mar 01 00:00:00 EST 2020

Exploration of the effect of salvianolate on myocardial infarction in rats based on tandem mass tags.

European journal of pharmacology

Chen C,Zhu P,Yu H,Huang B,Gui M,Lin X,Bai Y

Tue Dec 15 00:00:00 EST 2020

Inhibition of perivascular mast cell activation is involved in the atheroprotective effect of rosiglitazone in apolipoprotein E-deficient mice.

Biochemical and biophysical research communications

Li Q,Xiao Y,Lu G,Xie D,Zhai Y,Zhang J,Li J,Gao X

Tue Nov 05 00:00:00 EST 2019

Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.

Scientific reports

Meyer N,Woidacki K,Knöfler M,Meinhardt G,Nowak D,Velicky P,Pollheimer J,Zenclussen AC

Wed Mar 22 00:00:00 EDT 2017

Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.

Scientific reports

Meyer N,Woidacki K,Knöfler M,Meinhardt G,Nowak D,Velicky P,Pollheimer J,Zenclussen AC

Wed Mar 22 00:00:00 EDT 2017

Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.

Scientific reports

Meyer N,Woidacki K,Knöfler M,Meinhardt G,Nowak D,Velicky P,Pollheimer J,Zenclussen AC

Wed Mar 22 00:00:00 EDT 2017

Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device.

Cell cycle (Georgetown, Tex.)

Shi H,Jiang H,Wang L,Cao Y,Liu P,Xu X,Wang Y,Sun L,Niu H

Tue Aug 30 00:00:00 EDT 2016

Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device.

Cell cycle (Georgetown, Tex.)

Shi H,Jiang H,Wang L,Cao Y,Liu P,Xu X,Wang Y,Sun L,Niu H

Tue Aug 30 00:00:00 EDT 2016

Expression of recombinant human mast cell chymase with Asn-linked glycans in glycoengineered Pichia pastoris.

Protein expression and purification

Smith ET,Perry ET,Sears MB,Johnson DA

Wed Oct 01 00:00:00 EDT 2014

Expression of recombinant human mast cell chymase with Asn-linked glycans in glycoengineered Pichia pastoris.

Protein expression and purification

Smith ET,Perry ET,Sears MB,Johnson DA

Wed Oct 01 00:00:00 EDT 2014

The circadian clock is functional in eosinophils and mast cells.

Immunology

Baumann A,Gönnenwein S,Bischoff SC,Sherman H,Chapnik N,Froy O,Lorentz A

Sun Dec 01 00:00:00 EST 2013

The circadian clock is functional in eosinophils and mast cells.

Immunology

Baumann A,Gönnenwein S,Bischoff SC,Sherman H,Chapnik N,Froy O,Lorentz A

Sun Dec 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: Alpha-chymase; Chymase; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; chymase, heart; chymase, mast cell; mast cell; Mast cell chymase 1; Mast cell protease 3; Mast cell protease 5; Mast cell protease I; Mast cell protease III; mMCP-5; rMCP-3; rMCP-5; rMCP-III

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Gene Aliases: chymase; CMA1; CYH; CYM; Mcp-5; MCP3P; Mcp5; Mcpt3; Mcpt5; MCT1; MMCP-5

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UniProt ID: (Human) P23946, (Rat) P50339, (Mouse) P21844

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Entrez Gene ID: (Human) 1215, (Rat) 25627, (Mouse) 17228

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Function(s)

endopeptidase activity serine-type endopeptidase activity serine-type peptidase activity peptide binding peptidase activity hydrolase activity protease serine protease protein modifying enzyme

Process(es)

angiotensin maturation immune response protein processing extracellular matrix disassembly midbrain development positive regulation of angiogenesis interleukin-1 beta biosynthetic process regulation of inflammatory response cellular response to glucose stimulus proteolysis peptide metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Mast Cell Chymase Polyclonal Antibody

Mast Cell Chymase Antibody (18189-1-AP) in IHC (P)

Product Details

18189-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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