Invitrogen

Metadherin Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

13 Published Figures

10 References

View all (45) Metadherin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Metadherin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 21

Metadherin Antibody (40-6400) in ICC/IF

Immunofluorescence analysis of Metadherin was done on 70% confluent log phase U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Metadherin Rabbit Polyclonal Antibody (Product # 40-6400) at 1.5 µg/mL and incubated for 3 hours at room temperature an... View More

Metadherin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Metadherin Antibody in Western Blot (WB)

Metadherin Antibody in Immunohistochemistry (IHC)

Metadherin Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Metadherin Antibody in Immunoprecipitation (IP)

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Product Details

40-6400

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Raised against the C-term of human Metadherin.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533474

Target Information

Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression of astrocyte-elevated gene-1 indicates prognostic value of fluoropyrimidine-based adjuvant chemotherapy in resectable stage III colorectal cancer.

Pathology international

Lin LW,Lai PS,Chen YY,Chen CY

40-6400 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to demonstrate that AEG-1 expression and tumor grade are potential prognostic factors for recurrence and survival in patients with stage III CRC receiving adjuvant fluoropyrimidine-based chemotherapy.

Mon Nov 01 00:00:00 EDT 2021

Expression of astrocyte-elevated gene-1 indicates prognostic value of fluoropyrimidine-based adjuvant chemotherapy in resectable stage III colorectal cancer.

Pathology international

Lin LW,Lai PS,Chen YY,Chen CY

Mon Nov 01 00:00:00 EDT 2021

MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells.

Biological research

Cao D,Zhu H,Zhao Q,Huang J,Zhou C,He J,Liang Y

40-6400 was used in Immunohistochemistry and Western Blotting to investigate the role of miR-128 in breast cancer.

Tue Sep 29 00:00:00 EDT 2020

MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells.

Biological research

Cao D,Zhu H,Zhao Q,Huang J,Zhou C,He J,Liang Y

40-6400 was used in Immunohistochemistry and Western Blotting to investigate the role of miR-128 in breast cancer.

Tue Sep 29 00:00:00 EDT 2020

MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells.

Biological research

Cao D,Zhu H,Zhao Q,Huang J,Zhou C,He J,Liang Y

40-6400 was used in Immunohistochemistry and Western Blotting to investigate the role of miR-128 in breast cancer.

Tue Sep 29 00:00:00 EDT 2020

MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells.

Biological research

Cao D,Zhu H,Zhao Q,Huang J,Zhou C,He J,Liang Y

40-6400 was used in Immunohistochemistry and Western Blotting to investigate the role of miR-128 in breast cancer.

Tue Sep 29 00:00:00 EDT 2020

Astrocyte-elevated gene-1 confers resistance to pemetrexed in non-small cell lung cancer by upregulating thymidylate synthase expression.

Oncotarget

Chen CY,Chen YY,Chen JJW,Chen KY,Ho CC,Shih JY,Chang YL,Yu CJ,Yang PC

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Fri Sep 22 00:00:00 EDT 2017

MiR-630 suppresses breast cancer progression by targeting metadherin.

Oncotarget

Zhou CX,Wang CL,Yu AL,Wang QY,Zhan MN,Tang J,Gong XF,Yin QQ,He M,He JR,Chen GQ,Zhao Q

40-6400 was used in western blot to assess the power of MiR-630 in supressing breast cancer through metadherin

Tue Jan 12 00:00:00 EST 2016

MiR-630 suppresses breast cancer progression by targeting metadherin.

Oncotarget

Zhou CX,Wang CL,Yu AL,Wang QY,Zhan MN,Tang J,Gong XF,Yin QQ,He M,He JR,Chen GQ,Zhao Q

40-6400 was used in western blot to assess the power of MiR-630 in supressing breast cancer through metadherin

Tue Jan 12 00:00:00 EST 2016

Astrocyte elevated gene-1 interacts with β-catenin and increases migration and invasion of colorectal carcinoma.

Molecular carcinogenesis

Zhang F,Yang Q,Meng F,Shi H,Li H,Liang Y,Han A

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Thu Aug 01 00:00:00 EDT 2013

Astrocyte elevated gene-1 interacts with β-catenin and increases migration and invasion of colorectal carcinoma.

Molecular carcinogenesis

Zhang F,Yang Q,Meng F,Shi H,Li H,Liang Y,Han A

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Thu Aug 01 00:00:00 EDT 2013

Astrocyte elevated gene-1 interacts with β-catenin and increases migration and invasion of colorectal carcinoma.

Molecular carcinogenesis

Zhang F,Yang Q,Meng F,Shi H,Li H,Liang Y,Han A

Published figure using Metadherin polyclonal antibody (Product # 40-6400) in Immunohistochemistry

Thu Aug 01 00:00:00 EDT 2013

Pathologically decreased miR-26a antagonizes apoptosis and facilitates carcinogenesis by targeting MTDH and EZH2 in breast cancer.

Carcinogenesis

Zhang B,Liu XX,He JR,Zhou CX,Guo M,He M,Li MF,Chen GQ,Zhao Q

Sat Jan 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC

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Gene Aliases: 2610103J23Rik; 3D3; 3D3/Lyric; AEG-1; AEG1; AV353288; D8Bwg1112e; LYRIC; LYRIC/3D3; MTDH

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UniProt ID: (Human) Q86UE4, (Mouse) Q80WJ7

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Entrez Gene ID: (Human) 92140, (Mouse) 67154

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Function(s)

RNA polymerase II transcription factor binding transcription coactivator activity double-stranded RNA binding protein binding poly(A) RNA binding NF-kappaB binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter positive regulation of autophagy lipopolysaccharide-mediated signaling pathway negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of angiogenesis positive regulation of NF-kappaB transcription factor activity positive regulation of protein kinase B signaling bicellular tight junction assembly

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Metadherin Polyclonal Antibody

Metadherin Antibody (40-6400) in ICC/IF

Product Details

40-6400

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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