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Metadherin Antibody (PA5-85097) in ICC/IF
Metadherin Polyclonal Antibody detects LYRIC protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: LYRIC protein stained by Metadherin Polyclonal Antibody (Product # PA5-85097) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-85097
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human LYRIC.
Conjugate
Form
Liquid
Concentration
0.69 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2792245
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (91%), Rat (91%), Pig (93%), Rhesus Monkey (98%), Bovine (94%).
Positive Control: rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC
Gene Aliases: 2610103J23Rik; 3D3; 3D3/Lyric; AEG-1; AEG1; AV353288; D8Bwg1112e; LYRIC; LYRIC/3D3; MTDH
UniProt ID: (Human) Q86UE4, (Mouse) Q80WJ7, (Rat) Q9Z1W6
Entrez Gene ID: (Human) 92140, (Mouse) 67154, (Rat) 170910
negative regulation of transcription from RNA polymerase II promoter
positive regulation of autophagy
lipopolysaccharide-mediated signaling pathway
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of angiogenesis
positive regulation of NF-kappaB transcription factor activity
positive regulation of protein kinase B signaling
bicellular tight junction assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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