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Invitrogen
Metadherin Monoclonal Antibody (2F11C3)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 7
Metadherin Antibody (MA5-15564) in ICC/IF
Immunofluorescence analysis of MTDH was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Metadherin Monoclonal Antibody (2F11C3) (Product # MA5-15564, 1:100) in 0.1% BSA, incubated at 4 degree celsius overni... View More
Product Details
MA5-15564
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2F11C3
Immunogen
Purified recombinant fragment of human Metadherin expressed in E. Coli.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_10985221
Product Specific Information
MA5-15564 targets Metadherin in indirect ELISA, FACS, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15564 immunogen is purified recombinant fragment of human Metadherin expressed in E. Coli.
MA5-15564 detects Metadherin which has a predicted molecular weight of approximately 64kDa.
Target Information
Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain; blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3D3/LYRIC; AEG-1; astrocyte elevated gene 1; Astrocyte elevated gene-1 protein; LYRIC/ MTDH; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; Protein LYRIC
Gene Aliases: 3D3; AEG-1; AEG1; LYRIC; LYRIC/3D3; MTDH
UniProt ID: (Human) Q86UE4
Entrez Gene ID: (Human) 92140
negative regulation of transcription from RNA polymerase II promoter
positive regulation of autophagy
lipopolysaccharide-mediated signaling pathway
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of angiogenesis
positive regulation of NF-kappaB transcription factor activity
positive regulation of protein kinase B signaling
bicellular tight junction assembly
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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