Invitrogen

Mitochondria Membrane Integrity Antibody Cocktail

2 Published Figures
1 Reference
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

45-7799

Applications
Tested Dilution
Publications

Western Blot (WB)

10.2 µg/mL
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Product Specifications

Species Reactivity

Bovine, Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Mouse / Ig

Class

Cocktail

Type

Antibody

Clone

Cocktail

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2.37 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2533833

Product Specific Information

Mitochondria Membrane Integrity cocktail of monoclonal antibodies specific to human, bovine, mouse, rat mitochondria membranes. This antibody is validated for use in Western Blot, and can be used to assess mitochondrial membrane integrity during and after purification.

Contains:

50 µg of Porin clone #2B12AF12 - Human VDAC1. Concentration 2 µg/mL.

50 µg of Cyclophilin D clone #E11AE12BD4 - Rat CypD/PPIF. Concentration 2 µg/mL.

50 µg of C-III-Core 1 clone # 16D10AD9AH5 - Bovine Heart Complex II/III. Concentration 2 µg/mL.

5 µg of Cytochrome C clone #37BA11 - Human Cytochrome c. Concentration 0.1 µg/mL.

100 µg of C-V-a clone #7H10BD4F9 - Bovine Complex V. Concentration 4 µg/mL.

This antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of 10.2 µg/mL for use in Western blot applications.

Target Information

Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. It was discovered in the mitochondrial outer membrane. It also is expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. For example, VDAC contitutes a major pathway by which metabolites such as ADP/ATP, succinate and citrate are exchanged between the cytosol and mitochondria. And VDAC1 in the plasma membrane establishes a novel level of apoptosis regulation putatively via its redox activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 16D10AD9AH5; 20B12AF2; 37BA11; 7H10BD4F9; BR1-VDAC; Brain-derived voltage-dependent anion channel 1; Complex III subunit 4; Complex III subunit IV; cyclophilin 3; Cyclophilin D; Cyclophilin F; CyP-D; CyP-M; Cytochrome b-c1 complex subunit 4; Cytochrome c; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; Cytochrome c-1; Cytochrome c1, heme protein, mitochondrial; E11AE12BD4; Mitochondrial cyclophilin; mitochondrial Cyclophilin D; mVDAC1; mVDAC5; Non-selective voltage-gated ion channel VDAC1; Outer mitochondrial membrane protein porin 1; Peptidyl-prolyl cis-trans isomerase F, mitochondrial; peptidyl-prolyl cis-trans isomerase, mitochondrial; peptidylprolyl isomerase F; Plasmalemmal porin; Porin 31HL; Porin 31HM; PPIase F; Rotamase F; rVDAC1; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; VDAC-1; VDAC-5; Voltage-dependent anion-selective channel protein 1; Voltage-dependent anion-selective channel protein 5

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Gene Aliases: 2610002H19Rik; AA408921; AL033343; AW457192; CYC; CYC1; CYCS; CYCSA; CyP-D; CyP-F; CyP-M; CYP3; CypD; cytc; HCS; MC3DN6; PORIN; PPIase; PPIF; THC4; UQCR4; VDAC; VDAC-1; VDAC1; Vdac5

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UniProt ID: (Human) P08574, (Bovine) P00125, (Human) P99999, (Bovine) P62894, (Mouse) Q9D0M3, (Mouse) P62897, (Rat) P62898, (Human) P30405, (Bovine) P30404, (Rat) P29117, (Mouse) Q99KR7, (Human) P21796, (Bovine) P45879, (Mouse) Q60932, (Rat) Q9Z2L0

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Entrez Gene ID: (Human) 1537, (Bovine) 512500, (Human) 54205, (Bovine) 510767, (Rat) 300047, (Mouse) 66445, (Mouse) 13063, (Rat) 25309, (Human) 10105, (Bovine) 414346, (Rat) 282819, (Mouse) 105675, (Human) 7416, (Bovine) 282119, (Mouse) 22333, (Rat) 83529

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Function(s)
heme binding electron transporter, transferring electrons from CoQH2-cytochrome c reductase complex and cytochrome c oxidase complex activity metal ion binding protein serine/threonine phosphatase activity protein binding electron carrier activity molecular_function iron ion binding lipid binding peptidyl-prolyl cis-trans isomerase activity cyclosporin A binding peptide binding isomerase activity voltage-gated anion channel activity porin activity protein kinase binding protein complex binding ion channel binding nucleotide binding anion channel activity voltage-gated ion channel chaperone
Process(es)
mitochondrial electron transport, ubiquinol to cytochrome c response to glucagon response to reactive oxygen species mitochondrial electron transport, cytochrome c to oxygen protein dephosphorylation mitochondrion organization activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c cellular respiration intrinsic apoptotic signaling pathway apoptotic process transport oxidation-reduction process apoptotic DNA fragmentation hydrogen peroxide metabolic process ATP synthesis coupled electron transport response to ischemia response to oxidative stress negative regulation of hydrogen peroxide biosynthetic process glial cell apoptotic process positive regulation of cellular respiration protein peptidyl-prolyl isomerization protein folding apoptotic mitochondrial changes regulation of proton-transporting ATPase activity, rotational mechanism regulation of necrotic cell death negative regulation of ATPase activity negative regulation of apoptotic process regulation of mitochondrial membrane permeability necroptotic process cellular response to hydrogen peroxide cellular response to arsenic-containing substance cellular response to calcium ion positive regulation of release of cytochrome c from mitochondria negative regulation of release of cytochrome c from mitochondria negative regulation of oxidative phosphorylation regulation of mitochondrial membrane permeability involved in programmed necrotic cell death negative regulation of oxidative phosphorylation uncoupler activity negative regulation of intrinsic apoptotic signaling pathway mitochondrial outer membrane permeabilization involved in programmed cell death regulation of apoptotic process programmed cell death behavioral fear response anion transport mitochondrial calcium ion transport neuron-neuron synaptic transmission learning viral process macroautophagy epithelial cell differentiation anion transmembrane transport regulation of mitophagy regulation of anion transmembrane transport negative regulation of reactive oxygen species metabolic process inorganic anion transport ion transport chemical synaptic transmission regulation of anion transport transmembrane transport ion transmembrane transport regulation of ion transmembrane transport
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