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Invitrogen
MnSOD Monoclonal Antibody (MnS-1), FITC, eBioscience™
Product Details
BMS122FI
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MnS-1
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
10 µL/Test
Purification
Affinity chromatography
Storage buffer
TBS, pH 7.2-7.4, with 1% BSA
Contains
0.02% sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10609345
Product Specific Information
Description: Recognizes human Mn SOD. Cross-reacts with rat and rabbit Mn SOD.
The antibody can be used to stain both indirectly or directly acetone-fixed cryostat sections or cell smears.
BMS122 is suitable for Western blot application. Under reducing conditions/SDS electrophoresis using BMS122 a 25 kDa band will be recognized (monomer of MnSOD protein).
The native form of the protein with a molecular weight of 80 kDa will be recognized under non-reducing conditions.
Human MnSOD is a tetrameric enzyme with four identical subunits each harboring a Mn+3 atom. MnSOD catalyzes the dismutation of two molecules of superoxide anion into water and hydrogen peroxide.
Purity: >95%.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Target Information
Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD-1), mitochondrial manganese SOD (Mn-SOD/SOD-2) and extracellular Cu, Zn-SOD (EC-SOD/SOD-3). SOD1 is found in all eukaryotic species as a homodimeric 32 kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80 kDa tetrameric enzyme SOD2, is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin-binding multimer of disulfide-linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase (CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: indophenoloxidase B; IPO B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; superoxide dismutase; Superoxide dismutase [Mn], mitochondrial
Gene Aliases: IPO-B; IPOB; Mn-SOD; MNSOD; MVCD6; SOD2
UniProt ID: (Human) P04179
Entrez Gene ID: (Human) 6648
response to reactive oxygen species
response to superoxide
age-dependent response to reactive oxygen species
release of cytochrome c from mitochondria
vasodilation by acetylcholine involved in regulation of systemic arterial blood pressure
regulation of transcription from RNA polymerase II promoter
superoxide metabolic process
regulation of blood pressure
negative regulation of cell proliferation
removal of superoxide radicals
oxygen homeostasis
negative regulation of neuron apoptotic process
protein homotetramerization
oxidation-reduction process
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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