Invitrogen

Mouse Hematopoietic Lineage Antibody Cocktail, FITC, eBioscience™

Mouse Hematopoietic Lineage Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

Mouse Hematopoietic Lineage Antibody (22-7770-72) in Flow

C57Bl/6 bone marrow cells were stained with the Mouse Hematopoietic Lineage FITC Cocktail, Anti-Mouse CD117 PE (Product # 12-1171-82) and Anti-Mouse CD34 eFluor® 660 (Product # 50-0341-82). Total viable cells, as determined by 7-AAD (Product # 00-6993-50), were used for analysis. Events displayed on the right plot are gated on lineage negative events, as shown on the left histogram.

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Product Details

22-7770-72

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

20 µL/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat

Class

Cocktail

Type

Antibody

Clone

17A2, RA3-6B2, M1/70, TER-119, RB6-8C5

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

20 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2644066

Product Specific Information

Description: The Mouse Hematopoietic Lineage FITC Cocktails contains FITC-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on the expression of cell surface antigens.

This cocktail contains the following antibodies:

Anti-Mouse CD3 (17A2) FITC

Anti-Mouse CD45R (B220) (RA3-6B2) FITC

Anti-Mouse CD11b (M1/70) FITC

Anti-Mouse TER-119 (TER-119) FITC

Anti-Mouse Ly-G6 (Gr-1) (RB6-8C5) FITC

Applications Reported: The Mouse Hematopoietic Lineage FITC Cocktail has been reported for use in flow cytometric analysis.

Applications Tested: The Mouse Hematopoietic Lineage FITC Cocktail has been pre-titrated and tested by flow cytometric analysis of C57Bl/6 bone marrow cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Target Information

Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Product References

STS1 and STS2 Phosphatase Inhibitor Baicalein Enhances the Expansion of Hematopoietic and Progenitor Stem Cells and Alleviates 5-Fluorouracil-Induced Myelosuppression.

International journal of molecular sciences

Li N,Wang Y,Wang A,Zhang J,Jia C,Yu C,Song Z,Wang S,Liu L,Yi J,Bao Y,Huang Y,Sun L

22-7770-72 was used in Flow cytometry/Cell sorting to suggest BC as one of the small molecule candidates to stimulate HSPC expansion both in vitro and in vivo when needed in either physiologic or pathologic conditions, but also supports STS1/STS2 as potential therapeutic drug targets for HSPC expansion and hematopoietic injury recovery.

Fri Feb 03 00:00:00 EST 2023

Gasdermin D inhibition ameliorates neutrophil mediated brain damage in acute ischemic stroke.

Cell death discovery

Hu R,Liang J,Ding L,Zhang W,Wang Y,Zhang Y,Zhang D,Pei L,Liu X,Xia Z,Xu Y,Song B

22-7770-72 was used in Flow cytometry/Cell sorting to provide a new viewpoint on the immunological modulation of neutrophils after MCAO and suggest that suppression of GSDMD may relieve the neuroinflammatory load, thereby providing a potential treatment strategy for stroke.

Wed Feb 08 00:00:00 EST 2023

Deciphering the Efficacy and Mechanism of Astragalus membranaceus on High Altitude Polycythemia by Integrating Network Pharmacology and In Vivo Experiments.

Nutrients

Liu X,Zhang H,Yan J,Li X,Li J,Hu J,Shang X,Yang H

22-7770-72 was used in Flow cytometry/Cell sorting to discover if astragalus membranaceus (AM) could be a candidate for the prevention of high altitude polycythemia (HAPC) and its mechanism.

Wed Nov 23 00:00:00 EST 2022

Human gut bacterial metabolism drives Th17 activation and colitis.

Cell host & microbe

Alexander M,Ang QY,Nayak RR,Bustion AE,Sandy M,Zhang B,Upadhyay V,Pollard KS,Lynch SV,Turnbaugh PJ

22-7770-72 was used in Flow cytometry/Cell sorting to support a role for human gut bacterial metabolism in driving Th17-dependent autoimmunity.

Wed Jan 12 00:00:00 EST 2022

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

22-7770-72 was used in Flow Cytometry to indicate that the gut microbial metabolite PA can attenuate intestinal I/R injury by promoting the release of IL-13 from ILC2s via IL-33/ST2 signaling.

Tue Dec 21 00:00:00 EST 2021

Type 2 Innate Lymphoid Cells Accumulate in the Brain After Hypoxia-Ischemia but Do Not Contribute to the Development of Preterm Brain Injury.

Frontiers in cellular neuroscience

Zelco A,Rocha-Ferreira E,Nazmi A,Ardalan M,Chumak T,Nilsson G,Hagberg H,Mallard C,Wang X

22-7770 was used in Flow cytometry/Cell sorting to investigate if innate lymphoid cells, especially ILC2s, play a role in preterm brain injury.

Sat Apr 16 00:00:00 EDT 2022

CD137 Signaling Regulates Acute Colitis via RALDH2-Expressing CD11b^-CD103⁺ DCs.

Cell reports

Jin J,Jung IH,Moon SH,Jeon S,Jeong SJ,Sonn SK,Seo S,Lee MN,Song EJ,Kweon HY,Kim S,Kim TK,Kim J,Cho HR,Choi JH,Kwon B,Oh GT

22-7770 was used in Flow cytometry/Cell sorting to define a mechanism of paracrine immunoregulation operating between adjacent DC subsets in the intestine.

Tue Mar 24 00:00:00 EDT 2020

Manipulation of the tumor microenvironment by cytokine gene transfection enhances dendritic cell-based immunotherapy.

FASEB bioAdvances

Wijesekera DPH,Yuba E,De Silva NH,Watanabe SI,Tsukamoto M,Ichida C,Izawa T,Itoh K,Kanegi R,Hatoya S,Yamate J,Inaba T,Sugiura K

22-7770-72 was used in Flow Cytometry to manipulate the microenvironment so as to elicit activation of T helper type 1 (Th1) responses and the maturation of dendritic cells (DCs).

Wed Jan 01 00:00:00 EST 2020

A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion.

Immunity

Xia P,Wang S,Ye B,Du Y,Li C,Xiong Z,Qu Y,Fan Z

22-7770 was used in Flow cytometry/Cell sorting to study circular RNA-mediated maintenance of hematopoeitic stem cell homeostasis.

Tue Apr 17 00:00:00 EDT 2018

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

22-7770 was used in Flow cytometry/Cell sorting to investigate whether Dermatophagoides farinae-31 modulates airway epithelial cell functions, and increases the frequency of lung-resident group 2 innate lymphoid cells.

Thu Aug 17 00:00:00 EDT 2017

Caloric Restriction Leads to Browning of White Adipose Tissue through Type 2 Immune Signaling.

Cell metabolism

Fabbiano S,Suárez-Zamorano N,Rigo D,Veyrat-Durebex C,Stevanovic Dokic A,Colin DJ,Trajkovski M

22-7770 was used in Flow cytometry/Cell sorting to show that caloric restriction stimulates development of functional beige fat within the subcutaneous and visceral adipose tissue in mice.

Tue Sep 13 00:00:00 EDT 2016

Synergistically increased ILC2 and Th9 cells in lung tissue jointly promote the pathological process of asthma in mice.

Molecular medicine reports

Ying X,Su Z,Bie Q,Zhang P,Yang H,Wu Y,Xu Y,Wu J,Zhang M,Wang S,Xu H

22-7770 was used in Flow cytometry/Cell sorting to investigate the response state of lung tissue with regards to Th9 and innate lymphoid cells 2 in a mouse model of asthma.

Wed Jun 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: 17A2; Hematopoietic Lineage Cocktail; M1/70; RA3-6B2; RB6-8C5; TER-119

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