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Mouse IgG Isotype Control (10400C) in Ctrl
Lola29M/F molecular actions. a-hrobo1 promoter-luciferase reporter assays. a The robo1 genomic map (upper) and reporter schematics (lower). The genomic distance (in kb) is indicated on the top with the reference point 0, at which the robo1 genomic fragment was fused with the luciferase-coding sequence (blue box with an arrow). Below the thick line, the exon (box)-intron (thin line) organization of robo1 is ... View More
Product Details
10400C
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Isotype Control
Conjugate
Form
Liquid
Concentration
3 mg/mL
Purification
purified
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2532980
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; ImmunoglobulinG
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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