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Mouse IgG Isotype Control (31903) in Flow
Flow cytometry analysis of Synaptophysin was done on PC12 cells. The cells were fixed, permeabilized and stained with a Synaptophysin mouse monoclonal antibody (Product # MA1-213, red histogram) or Mouse IgG isotype control (Product # 31903, blue histogram) at a dilution of 1:500. Black histogram represents negative control unstained cell population. After incubation of the primary antibody on ice for an ho... View More
Product Details
31903
Applications
Tested Dilution
Immunohistochemistry (IHC)
Assay-dependent
Immunocytochemistry (ICC/IF)
Assay-dependent
Flow Cytometry (Flow)
1:500
Control (Ctrl)
Assay-Dependent
Blocking Assay (BLOCK)
Assay-dependent
Product Specifications
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Isotype Control
Conjugate
Form
Liquid
Concentration
5.05 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.6
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10959891
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Purity: Based on immunoelectrophoresis at an antigen concentration of 20 mg/mL, the pattern of precipitation against goat anti-mouse whole serum is the same as that against goat anti-mouse IgG, Fc fragment specific. No precipitin line was detected against goat anti-mouse IgA, alpha chain specific or goat anti-mouse IgM, µ chain specific.
This antibody is recommended for use in blocking assays, immunohistochemical, immunocytochemical and immunofluorescent applications.
Store product at 4°C until opened. After opening, dilute only enough material for a single day’s use.; store remainder at 4°C under sterile conditions.
Country of Origin: USA
Target Information
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody's species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; ImmunoglobulinG
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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