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Mouse IgG1 Isotype Control (MA5-14453) in Flow
Flow cytometry analysis of CD38 on Ramos cells. Cells were fixed, permeabilized, and stained with a CD38 monoclonal antibody (Product # MA1-182, blue histogram) or with a mouse IgG1 isotype control (Product # MA5-14453, red histogram) at a concentration of 1 µg/mL. After incubation with the primary antibody for 1 hour on ice, the cells were stained with a DyLight 488-conjugated goat anti-mouse IgG secondary... View More
Product Details
MA5-14453
Applications
Tested Dilution
Western Blot (WB)
1:50-1:100
Immunohistochemistry (Paraffin) (IHC (P))
1:200-1:400
Immunohistochemistry (Frozen) (IHC (F))
1:200-1:400
Flow Cytometry (Flow)
1 µg/mL
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Isotype Control
Clone
NCG01
Immunogen
Synthetic hapten which is normally not present in humans or animals
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10943239
Product Specific Information
MA5-14453 reacts in ELISA, flow cytometry, gel shift, immunofluorescence, immunohistochemistry (paraffin and frozen), immunoprecipitation, and western blot analysis.
Target Information
Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG1 is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Immunoglobulin G; Immunoglobulin G1; ImmunoglobulinG; ImmunoglobulinG1
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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