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Product Details
PA5-33207
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Isotype Control
Immunogen
Human T lymphocytes
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Protein A
Storage buffer
PBS with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2550623
Product Specific Information
PA5-33207 is suitable for use as a matched isotype negative control reagent to assess the level of non-specific binding of mouse IgG2a monoclonal antibodies in rat cells. This antibody has been tested and found to be negative on the following rat cells €“ peripheral blood leucocytes, thymocytes, splenocytes and macrophages.
PA5-33207 recognizes the human CD45 antigen and therefore human leucocytes may be used as a positive control for this product. This antibody is not suitable for use as a negative control on human tissues.
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
Mouse IgG2a is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the second most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2a is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2a is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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