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Invitrogen
Alpaca anti-Mouse IgG2b Nano (VHH) Recombinant Secondary Antibody, Alexa Fluor™ 488
FIGURE: 1 / 3
Mouse IgG2b Nano (VHH) Secondary Antibody (SA5-10334) in ICC/IF
Immunofluorescence analysis in HeLa cells. Samples were incubated with mouse IgG1 anti-COX4 (green); mouse IgG2b anti-Tubulin (magenta); rabbit anti-Lamin (yellow) primary antibodies and followed by Mouse IgG2b (Fc-specific VHH) secondary antibody (Product # SA5-10335), alpaca anti-mouse IgG1 VHH Alexa Fluor 488; alpaca anti-mouse IgG2b VHH Alexa Fluor 647; and alpaca anti-rabbit IgG VHH Alexa Fluor 568.
Product Details
SA5-10334
Applications
Tested Dilution
Western Blot (WB)
1:1,000
Immunocytochemistry (ICC/IF)
1:1,000
Dot blot (DB)
Assay-dependent
Immunomicroscopy (IM)
1:1,000
Product Specifications
Host/Isotype
Alpaca
/ IgG2b
Expression System
E. coli
Class
Recombinant Monoclonal
Type
Secondary Antibody
Clone
CTK0105/CTK0106
Immunogen
Purified mouse IgG2b
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2868381
Target
IgG2b
Antibody Form
VHH
Product Specific Information
Biclonal nanobodies specific for mouse IgG2b Fc. CTK0105: KD = 5 nM, CTK0106: KD = 0.2 nM
Non Cross-Reactivity includes: Rabbit, rat, goat, sheep, guinea pig, human, and macaque serum and to mouse IgG1, IgG2a, IgG2c, and IgG3.
Rapid workflow: Simultaneous incubation of mouse IgG2b primary antibody and Alpaca anti-mouse IgG2b Nano (VHH) recombinant secondary antibody.
Offered through a partnership with ChromoTek
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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