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Invitrogen
Mouse IgG2b kappa Isotype Control (eBMG2b), eBioscience™
FIGURE: 1 / 12
Mouse IgG2b kappa Isotype Control (14-4732-82) in ICC/IF
Immunofluorescent analysis of transgenic SaCas9 expression (green) in A549 cells (60% confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at 37 C and blocked with 10% Normal Donkey Serum in PBS with 0.1% Triton X-100 for 1 hour at room temperature. Cells were stained with 0.6 µg/mL SaCas9 Mouse Monoclonal Antibody... View More
Product Details
14-4732-82
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
Assay-Dependent
Immunocytochemistry (ICC/IF)
Assay-Dependent
Flow Cytometry (Flow)
Assay-Dependent
Control (Ctrl)
Assay-Dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBMG2b
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_470117
Product Specific Information
Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.
Applications Reported: This mouse IgG2b isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunocytochemistry, immunohistochemistry.
Applications Tested: This mouse IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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