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Invitrogen
Mouse IgG2b kappa Isotype Control (eBMG2b), Brilliant Violet™ 421, eBioscience™
FIGURE: 1 / 1
Mouse IgG2b kappa Isotype Control (404-4732-81) in Flow
Normal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and staining buffer (autofluorescence) (left) or 0.25 µg of Mouse IgG2b kappa Isotype Control, Brilliant Violet 421 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
404-4732-81
Applications
Tested Dilution
Flow Cytometry (Flow)
Assay-dependent
Control (Ctrl)
Assay-dependent
Product Specifications
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Isotype Control
Clone
eBMG2b
Conjugate
Excitation/Emission Max
406/423 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2925525
Product Specific Information
Description: The monoclonal mouse IgG2b kappa is useful as an isotype control immunoglobulin.
Applications Reported: This eBMG2b antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBMG2b antibody has been tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.
Brilliant Violet™ 421 (BV421) is a dye that emits at 423 nm and is intended for use on cytometers equipped with a violet (405 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.
Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser.
BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Target Information
Mouse IgG2b is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the third most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG2b is composed of two heavy chains and two light chains, and it is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity. It is also involved in the regulation of immune responses, including the activation of T cells and the inhibition of B cell activation. Mouse IgG2b is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry. It is also used in therapeutic applications, such as immunotherapy and vaccine development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: IgG2; Immunoglobulin G; Immunoglobulin G2; ImmunoglobulinG; ImmunoglobulinG2
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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