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Invitrogen
Mouse anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Human IgG (H+L) Cross-Adsorbed Secondary Antibody (31420) in WB
Western blot (non-reducing) was performed using Mouse anti-Human IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP (Product # 31420) and a 150 kDa band corresponding to Human IgG was observed in IM-9, ARH-77 and IM-9, ARH-77 conditioned medium8203 (CM) but not in Raji, Jurkat, Molt-4 and Jurkat CM which are known to have low expression. Whole cell lysate (30 µg) of MOLT-4 (Lane 1), Jurkat (Lane 2), Raji (Lan... View More
Product Details
31420
Applications
Tested Dilution
Western Blot (WB)
1:2,000 - 1:200,000
Immunohistochemistry (IHC)
1:500-1:5,000
Immunocytochemistry (ICC/IF)
1:500-1:5,000
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.6, with 15mg/mL BSA
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient
RRID
AB_228374
Target
IgG
Cross Adsorption
Against mouse serum proteins
Antibody Form
Whole Antibody
Product Specific Information
Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.
Product # 31420 has been successfully used in Western blot, and ICC applications.
Antibody Specificity: Based on immunoelectrophoresis, the antibody reacts with the heavy chains on human IgG and with light chains common to most human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody exhibits minimal inherent cross-reactivity to mouse serum proteins. It has been passed through solid-phase immunoadsorbent gel to minimize any actual cross-reaction with mouse serum proteins. However, these antibodies may cross-react with immunoglobulins from other species.
Restoration and Storage: Store product at 4°C until opened. Restore with 1.5 mL distilled water (0.8 mg/mL after restoration). Centrifuge product if it is not completely clear after standing for 1-2 hours at room temperature. To judge clarity, draw product into a pasteur pipette. Product may be stored for several weeks at 4°C as undiluted liquid. After dilution, do not use for more than one day. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.
Country of Origin: USA
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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