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Invitrogen
Mouse anti-Human IgG4 Fc Secondary Antibody, HRP
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
Human IgG4 Fc Secondary Antibody (MA1-82201) in WB
Western blot was performed Mouse anti-Human IgG4 Fc Secondary Antibody, HRP (Product # MA1-82201) and a 55 kDa band corresponding to Human IgG4 Heavy Chain was observed in IgG48203 but not in IgG1, IgG2, IgG3, IgA, IgM, IgE, Mouse IgG, IgM and Rabbit IgG8203. Purified protein (100 ng) of Human IgG1 (Lane 1), IgG2 (Lane 2), IgG3 (Lane 3), IgG4 (Lane 4), IgA (Lane 5), IgM (Lane 6), IgE (Lane 7), Mouse IgG (La... View More
Product Details
MA1-82201
Applications
Tested Dilution
Western Blot (WB)
1:2000
Immunohistochemistry (Paraffin) (IHC (P))
Assay-dependent
Immunohistochemistry (Frozen) (IHC (F))
1/40-1/80
ELISA (ELISA)
1/1000-1/20000
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Secondary Antibody
Clone
HP6025
Immunogen
Purified IgG4
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_933979
Target
IgG4
Antibody Form
Whole Antibody
Product Specific Information
MA1-82201 detects the Fc portion of human IgG4. No cross-reactivity is observed with human IgG1, IgG2, and IgG3 isotypes.
A suggested histology positive control is tonsil tissue.
This antibody reacts with Human, African green monkey, Cynomolgus monkey, Baboon, Chimpanzee and Rhesus Monkey IgG.
Mouse anti Human IgG4 antibody, clone HP6025 recognizes the heavy chain of human IgG4, at an epitope in the Fc region. No cross-reactivity is observed with IgG1, IgG2, IgG3, IgM, IgA (Jefferis et al. 1985).
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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