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Invitrogen
Mouse anti-Rat IgG (H+L) Secondary Antibody, Biotin, eBioscience™
FIGURE: 1 / 6
Rat IgG (H+L) Secondary Antibody (13-4813-85) in ICC/IF
Immunofluorescent microscopy of fixed and permeabilized A375 cells using 10 µg/mL of Anti-Human/Mouse IL-20 Receptor 2 (IL-20Rb) Purified (right) or Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (left) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and then Streptavidin FITC (Product # 11-4317-87).
Product Details
13-4813-85
Applications
Tested Dilution
Western Blot (WB)
Assay-Dependent
Immunohistochemistry (Paraffin) (IHC (P))
Assay-dependent
Immunohistochemistry (Frozen) (IHC (F))
Assay-Dependent
Immunocytochemistry (ICC/IF)
Assay-dependent
Flow Cytometry (Flow)
0.25 µg/test
ELISA (ELISA)
Assay-Dependent
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_466659
Target
IgG
Antibody Form
Whole Antibody
Product Specific Information
Description: This antibody will recognize the light and heavy chains of other rat immunoglobulins. Reactions to non-immunoglobulin rat serum proteins have not been detected.
Applications Reported: In conjunction with enzyme- or fluorochrome-conjugated Streptavidin (SAV) or Avidin (AV), this polyclonal antibody can be used for detection of purified rat immunoglobulins (IgG) in ELISA, immunoblotting (WB), immunohistochemical staining and flow cytometric analysis.
Applications Tested: Biotin Anti-Rat IgG has been tested by flow cytometric analysis to detect purified rat monoclonal antibodies. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, horse and mouse serum proteins, but may cross-react with immunoglobµLins from other species.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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