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Invitrogen
Mouse anti-Rat IgG1 Secondary Antibody, APC, eBioscience™
FIGURE: 1 / 2
Rat IgG1 Secondary Antibody (17-4812-82) in Flow
Staining of C57Bl/6 splenocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and Anti-Mouse CD25 Purified (Product # 14-0251-86), followed by staining with 0.125 µg of Anti-Rat IgG1 APC. Cells in the lymphocyte gate were used for analysis. Quadrant lines were based on the isotype control.
Product Details
17-4812-82
Applications
Tested Dilution
Flow Cytometry (Flow)
0.25 µg/test
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Secondary Antibody
Clone
R1-12D10
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573210
Target
IgG1
Antibody Form
Whole Antibody
Product Specific Information
Description: The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG1.
Applications Reported: This R1-12D10 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This R1-12D10 antibody has been tested by flow cytometric analysis of cells stained with a rat IgG1 primary. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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