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Invitrogen
Mouse anti-Rat IgG1 Secondary Antibody, eFluor™ 615, eBioscience™
FIGURE: 1 / 1
Rat IgG1 Secondary Antibody (42-4812-82) in IHC (F)
Immunohistochemistry of frozen mouse intestine stained with 2-5 µg/mL Rat IgG1 K Isotype Control Purified (left) or 2.5 µg/mL Anti-Mouse Lyve-1 Purified (right) followed by 5 µg/mL Anti-Rat IgG1 eFluor® 615. Nuclei are stained with DAPI.
Product Details
42-4812-82
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
10 µg/mL
Immunohistochemistry (Frozen) (IHC (F))
10 µg/mL
Immunocytochemistry (ICC/IF)
Assay-Dependent
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Secondary Antibody
Clone
R1-12D10
Conjugate
Excitation/Emission Max
595/614 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573659
Target
IgG1
Antibody Form
Whole Antibody
Product Specific Information
Description: The R1-12D10 monoclonal antibody recognizes rat IgG1 antibodies and can be used as a second step reagent in microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG1.
Applications Reported: This R1-12D10 antibody has been reported for use in microscopy, immunohistochemical staining, and immunocytochemistry.
Applications Tested: This R1-12D10 antibody has been tested by immunohistochemistry on frozen mouse tissue and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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