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Invitrogen
Mouse anti-Rat IgG2a Secondary Antibody, eFluor™ 615, eBioscience™
FIGURE: 1 / 1
Rat IgG2a Secondary Antibody (42-4817-80) in IHC (F)
Immunohistochemistry on frozen mouse spleen with no primary antibody (left) or 1 µg/mL Anti-Human/ Mouse CD45R (B220) Purified (clone RA3-6B2, a rat IgG2a antibody) (right) followed by 10 µg/mL Anti-Rat IgG2a eFluor® 615. Nuclei are counterstained with DAPI.
Product Details
42-4817-80
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
10 µg/mL
Immunohistochemistry (Frozen) (IHC (F))
10 µg/mL
Immunocytochemistry (ICC/IF)
10 µg/mL
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Secondary Antibody
Clone
r2a-21B2
Conjugate
Excitation/Emission Max
595/614 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11151338
Target
IgG2a
Antibody Form
Whole Antibody
Product Specific Information
Description: The monoclonal antibody r2a-21B2 recognizes Rat IgG2a antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2a or any mouse isotype antibodies.
Applications Reported: This R2a-21B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.
Applications Tested: This R2a-21B2 antibody has been tested by immunocytochemistry and immunohistochemistry using a rat primary antibody at 10 µg/mL. This product has not been validated for flow cytometric analysis.
Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.
Excitation: 595 nm; Emission: 615 nm.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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