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Invitrogen
Mouse anti-Rat IgG2b Secondary Antibody, Biotin, eBioscience™
FIGURE: 1 / 1
Rat IgG2b Secondary Antibody (13-4815-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human tonsil tissue using 20 µg/mL of Rat IgG2b K Isotype Control Purified (left) or 20 µg/mL of Anti-Mouse Granzyme B Purified (right) followed by 10 µg/mL of Anti-Rat IgG2b Biotin, Streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin.
Product Details
13-4815-82
Applications
Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
10 µg/mL
Immunohistochemistry (Frozen) (IHC (F))
10 µg/mL
Immunocytochemistry (ICC/IF)
Assay-Dependent
Product Specifications
Species Reactivity
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Secondary Antibody
Clone
R2B-7C3
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572792
Target
IgG2b
Antibody Form
Whole Antibody
Product Specific Information
Description: The monoclonal antibody R2B-7C3 recognizes Rat IgG2b antibodies and can be used as a second step reagent in flow cytometry and microscopy. The monoclonal does not recognize other rat isotypes nor does it crossreact to mouse IgG2b or any mouse isotype antibodies.
Applications Reported: This R2B-7C3 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: This R2B-7C3 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue sections that have been stained first with a rat IgG2b anti-human antibody. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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